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<p>Genome editing has emerged as a cardinal tool to dissect molecular processes in mammalian cells. Standard gene editing approaches have however been inefficient in resting, non-dividing immune cells such as CD4 T helper cells. By combining an <i>ex vivo</i> tonsil culture system with highly efficient, activation-neutral gene editing using CRISPR-Cas9 technology, immune cell interactions of CD4 T cells and HIV pathology can now be dissected with high precision in a lymphoid tissue context.</p>

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Genom-Editierung von CD4+-T-Zellen in der ex vivo-Tonsillenkultur

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年3月25日

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  • Katharina Morath,
  • Oliver T. Fackler

摘要

Genome editing has emerged as a cardinal tool to dissect molecular processes in mammalian cells. Standard gene editing approaches have however been inefficient in resting, non-dividing immune cells such as CD4 T helper cells. By combining an ex vivo tonsil culture system with highly efficient, activation-neutral gene editing using CRISPR-Cas9 technology, immune cell interactions of CD4 T cells and HIV pathology can now be dissected with high precision in a lymphoid tissue context.