Absolute Proteinquantifizierung erlaubt Einblicke in den Proteintransport

With the help of methodological adaptations and suitable protein standards, mass spectrometry can determine the number of protein molecules per cell. This article describes recent developments in absolute protein quantification extending the method to analytically challenging proteins from the cell membrane or the extracellular medium. Finally, the integration of such data leads to information on the speed of protein transport and thus provides new insights into bacterial cell physiology.