Quantifizierung von Transkription und Translation von Riboschaltern
摘要
Riboswitches change their secondary structure upon ligand binding, sending a signal that triggers transcriptional or translational gene regulation. Our dual-readout assay allows the simultaneous detection of ligand-dependent changes in transcription and translation. The cell-free assay utilizes the parallel detection of the fluorescent Mango (IV) aptamer downstream of the transcribed mRNA and GFP fluorescence to quantify transcription and translation products in the presence or absence of ligand.