Elektrische Charakterisierung von Eizellen mit mikrofluidischen Systemen
摘要
In in vitro fertilization, the status of the zona pellucida is of pivotal interest to determine the window of best fertilization. In this window, the zona pellucida becomes soft, so that a sperm can penetrate. Two lab-on-chip systems will be introduced which were developed to monitor the status of the zona pellucida of mouse oocytes with electrical impedance spectroscopy. The combination of microfluidic systems and impedance spectroscopy offer advantages of easy cell handling and observer-independent characterization.