Kleine Ursache, große Wirkung – den springenden DNA-Sequenzen auf der Spur
摘要
At present, the instability of recombinant bacterial production hosts remains a major issue in plasmid-facilitated large-scale industrial fermentations, such as amino acid production. Heterogeneous starter populations, together with the onset of production escape mechanisms in evolving populations, are significant contributors to this phenomenon. Here, we present a combined omics-approach that led to the elucidation of the underlying mechanisms that constrain microbial L-cysteine production in Escherichia coli. These findings give rise to novel strain engineering approaches.