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<p>Mitral regurgitation (MR) is a significant risk factor for heart failure, yet existing rodent models face limitations in invasiveness and reproducibility. This study established a novel percutaneous, ultrasound-guided rat model of MR to overcome these constraints. Thirty male Sprague–Dawley rats underwent echocardiography-guided mitral valve injury, allocated to severe MR (40–70% regurgitation), massive MR (&gt; 70%), or sham groups. Serial echocardiographic assessments were performed at baseline, 30&#xa0;min, and 2, 4, and 8&#xa0;weeks post-procedure, with histological validation of myocardial fibrosis. The technique achieved 100% success with no acute mortality and enabled precise titration of regurgitation severity. Both MR groups exhibited progressive, body size-independent cardiac remodeling. Speckle-tracking revealed basal-segment-predominant longitudinal strain reduction, correlating with histologically confirmed peri-annular fibrosis. Survival was significantly lower in massive MR (40% vs. 90%, <i>p</i> = 0.006). This minimally invasive model reliably replicates human MR pathophysiology with high reproducibility and precise severity control, providing a robust platform for mechanistic investigation.&#xa0;</p> Graphical Abstract <p></p>

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Ultrasound-Guided Percutaneous Puncture to Establish a Rat Model of Mitral Regurgitation: A Non-Thoracotomy Approach

  • 期刊论文

  • 发表日期：

    2026年2月25日

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  • Kangla Liao,
  • Qian Dong,
  • Chunhua Mo,
  • Guoli Yang,
  • Bi Huang,
  • Suxin Luo

摘要

Mitral regurgitation (MR) is a significant risk factor for heart failure, yet existing rodent models face limitations in invasiveness and reproducibility. This study established a novel percutaneous, ultrasound-guided rat model of MR to overcome these constraints. Thirty male Sprague–Dawley rats underwent echocardiography-guided mitral valve injury, allocated to severe MR (40–70% regurgitation), massive MR (> 70%), or sham groups. Serial echocardiographic assessments were performed at baseline, 30 min, and 2, 4, and 8 weeks post-procedure, with histological validation of myocardial fibrosis. The technique achieved 100% success with no acute mortality and enabled precise titration of regurgitation severity. Both MR groups exhibited progressive, body size-independent cardiac remodeling. Speckle-tracking revealed basal-segment-predominant longitudinal strain reduction, correlating with histologically confirmed peri-annular fibrosis. Survival was significantly lower in massive MR (40% vs. 90%, p = 0.006). This minimally invasive model reliably replicates human MR pathophysiology with high reproducibility and precise severity control, providing a robust platform for mechanistic investigation. 

Graphical Abstract