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<p>Coarctation of the aorta (CoA) can cause aortic obstruction, ischemia, and death. Resonance Raman Spectroscopy (RRS) measures tissue oxyhemoglobin (ShbO<sub>2</sub>) and mitochondrial redox state (3RMR) non-invasively. Metal wire was placed around the aorta of Sprague Dawley rats to generate a systolic blood pressure (SBP) gradient. RRS-ShbO<sub>2</sub> and 3RMR were measured pre- (hand) and post-obstruction (foot). In model 1 (<i>n</i> = 8), the gradient rapidly reached 120&#xa0;mmHg. In model 2 (<i>n</i> = 30), gradients of 20&#xa0;mmHg (<i>n</i> = 9) and 40&#xa0;mmHg (<i>n</i> = 12) were maintained for 2&#xa0;h. In model 1, foot-ShbO<sub>2</sub> and 3RMR changed significantly (<i>P</i> = 0.004 and <i>P</i> = 0.007) at SBP gradients&#xa0;of 80-mmHg or above. In model 2, the 40-mmHg gradient group showed significant declines in foot-ShbO2 (<i>P</i> = 0.014) and increases in 3RMR (<i>P</i> = 0.008)&#xa0;by 1 h. Foot-ShbO<sub>2</sub> and 3RMR correlated strongly with serum mixed venous saturation (ShbO<sub>2</sub>: r = 0.73, <i>P</i> &lt; 0.0001; 3RMR: r = -0.55, <i>P</i> &lt; 0.0001). RRS effectively detects ischemia caused by aortic obstruction in a CoA model.</p> Graphical Abstract <p></p>

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Resonance Raman Spectroscopy Detects Ischemia in Experimental Coarctation of the Aorta in a Rodent Model

  • 期刊论文

  • 发表日期：

    2025年2月19日

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  • Alyssa R. Thomas,
  • Kristen Lazelle,
  • Julia Garcia Mancebo,
  • Padraic Romfh,
  • John N. Kheir

摘要

Coarctation of the aorta (CoA) can cause aortic obstruction, ischemia, and death. Resonance Raman Spectroscopy (RRS) measures tissue oxyhemoglobin (ShbO2) and mitochondrial redox state (3RMR) non-invasively. Metal wire was placed around the aorta of Sprague Dawley rats to generate a systolic blood pressure (SBP) gradient. RRS-ShbO2 and 3RMR were measured pre- (hand) and post-obstruction (foot). In model 1 (n = 8), the gradient rapidly reached 120 mmHg. In model 2 (n = 30), gradients of 20 mmHg (n = 9) and 40 mmHg (n = 12) were maintained for 2 h. In model 1, foot-ShbO2 and 3RMR changed significantly (P = 0.004 and P = 0.007) at SBP gradients of 80-mmHg or above. In model 2, the 40-mmHg gradient group showed significant declines in foot-ShbO2 (P = 0.014) and increases in 3RMR (P = 0.008) by 1 h. Foot-ShbO2 and 3RMR correlated strongly with serum mixed venous saturation (ShbO2: r = 0.73, P < 0.0001; 3RMR: r = -0.55, P < 0.0001). RRS effectively detects ischemia caused by aortic obstruction in a CoA model.

Graphical Abstract