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<p>Bioconversion process suggests a sustainable and green approach to producing high-value compounds from food waste. In this study, pineapple crown (PC) were utilized as a feedstock for lactic acid (LA) production through enzymatic hydrolysis and fermentation. Potassium hydroxide (KOH) pretreatment was performed to improve the enzymatic digestibility (ED) of PC, and the effect of KOH concentration on PC to glucose conversion (PtGC) was investigated. As a result, the 2% KOH pretreatment exhibited the highest PtGC (22.18%), along with a glucan content of 74.57%, a solids recovery of 38.90%, and an ED of 69.53%. The use of PC hydrolysate pretreated with 2% KOH as a carbon source in fermentation with <i>Lacticaseibacillus rhamnosus</i> yielded approximately 18.90&#xa0;g/L of LA, with a conversion rate of 94.5%. This result was comparable to that obtained using a control medium containing reagent-grade glucose (approximately 19.05&#xa0;g/L LA with conversion rate of 95.3%). Mass balance analysis revealed that 221.8&#xa0;kg of glucose could be produced from 1,000&#xa0;kg of PC via an alkali pretreatment and enzymatic hydrolysis process, and that this glucose could be utilized to produce 209.6&#xa0;kg of LA. This study presents a platform concept that effectively contributes to the sustainable management of waste such as PC and the production of high-value compounds.</p>

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Alkali pretreatment of pineapple crown to enhance glucose conversion and the application of its hydrolysate in lactic acid fermentation

  • 期刊论文

  • 发表日期：

    2026年7月22日

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  • Seunghee Kim,
  • Seungeun Kim,
  • Sion Yoo,
  • Yoonsoo Lee,
  • Eunseon Jeong,
  • Yunseok Song,
  • Minji Kim,
  • Soeun Shin,
  • Han-Sol Kim,
  • Jang-Seu Ki,
  • Hah Young Yoo

摘要

Bioconversion process suggests a sustainable and green approach to producing high-value compounds from food waste. In this study, pineapple crown (PC) were utilized as a feedstock for lactic acid (LA) production through enzymatic hydrolysis and fermentation. Potassium hydroxide (KOH) pretreatment was performed to improve the enzymatic digestibility (ED) of PC, and the effect of KOH concentration on PC to glucose conversion (PtGC) was investigated. As a result, the 2% KOH pretreatment exhibited the highest PtGC (22.18%), along with a glucan content of 74.57%, a solids recovery of 38.90%, and an ED of 69.53%. The use of PC hydrolysate pretreated with 2% KOH as a carbon source in fermentation with Lacticaseibacillus rhamnosus yielded approximately 18.90 g/L of LA, with a conversion rate of 94.5%. This result was comparable to that obtained using a control medium containing reagent-grade glucose (approximately 19.05 g/L LA with conversion rate of 95.3%). Mass balance analysis revealed that 221.8 kg of glucose could be produced from 1,000 kg of PC via an alkali pretreatment and enzymatic hydrolysis process, and that this glucose could be utilized to produce 209.6 kg of LA. This study presents a platform concept that effectively contributes to the sustainable management of waste such as PC and the production of high-value compounds.