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<p>We have previously examined several lysine decarboxylases (LDCs) for high-level production of cadaverine in recombinant <i>Corynebacterium glutamicum</i>, in which LDCs from <i>Escherichia coli</i> K-12 and <i>Hafnia alvei</i> supported most efficient production of cadaverine. Since it is important to expand LDC repertoire for high-level cadaverine production, five more LDC candidates from <i>Obesumbacterium proteus, Enterobacteriaceae</i> bacterium<i>, Salmonella enterica, Edwardsiella tarda</i> and probiotic <i>E. coli</i> Nissle 1917 were examined in <i>C. glutamicum</i> PKC for comparative evaluation of their cadaverine production capacities. All LDCs were expressed under the strong H36 synthetic promoter (P<sub>H36</sub>). Among the tested candidates, the <i>E. coli</i> Nissle LDC (EcNLdcC) supported a higher concentration of cadaverine (13.8 g/L) in recombinant <i>C. glutamicum</i> PKC compared to the 11.4 g/L produced by the expression of the <i>H. alvei</i> LDC (HaLdcC) using the P<sub>H36</sub> promoter in our previous study. Furthermore, batch and fed-batch fermentations of <i>C. glutamicum</i> PKC expressing the <i>E. coli</i> Nissle <i>ldcC</i> resulted in the production of 35 g/L and 99.3 g/L of cadaverine, respectively, both of which were higher than those obtained by the expression of <i>H. alvei ldcC</i> gene. These findings not only demonstrate the superior industrial potential of EcNLdcC for high-level cadaverine production but also highlight its advantages of utilizing a non-pathogenic, Generally Recognized As Safe (GRAS)-status strain in industrial applications, establishing a robust microbial platform for sustainable bioproduction.</p>

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High-level production of cadaverine in recombinant Corynebacterium glutamicum by expressing Escherichia coli Nissle 1917 lysine decarboxylase

  • 期刊论文

  • 发表日期：

    2025年12月22日

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  • Jina Son,
  • Ji Yeon Lee,
  • Jong-Il Choi,
  • Yu Jung Sohn,
  • Ji Young Park,
  • Joonyoung Lee,
  • Ki Jun Jeong,
  • Jung Eun Yang,
  • Si Jae Park

摘要

We have previously examined several lysine decarboxylases (LDCs) for high-level production of cadaverine in recombinant Corynebacterium glutamicum, in which LDCs from Escherichia coli K-12 and Hafnia alvei supported most efficient production of cadaverine. Since it is important to expand LDC repertoire for high-level cadaverine production, five more LDC candidates from Obesumbacterium proteus, Enterobacteriaceae bacterium, Salmonella enterica, Edwardsiella tarda and probiotic E. coli Nissle 1917 were examined in C. glutamicum PKC for comparative evaluation of their cadaverine production capacities. All LDCs were expressed under the strong H36 synthetic promoter (PH36). Among the tested candidates, the E. coli Nissle LDC (EcNLdcC) supported a higher concentration of cadaverine (13.8 g/L) in recombinant C. glutamicum PKC compared to the 11.4 g/L produced by the expression of the H. alvei LDC (HaLdcC) using the PH36 promoter in our previous study. Furthermore, batch and fed-batch fermentations of C. glutamicum PKC expressing the E. coli Nissle ldcC resulted in the production of 35 g/L and 99.3 g/L of cadaverine, respectively, both of which were higher than those obtained by the expression of H. alvei ldcC gene. These findings not only demonstrate the superior industrial potential of EcNLdcC for high-level cadaverine production but also highlight its advantages of utilizing a non-pathogenic, Generally Recognized As Safe (GRAS)-status strain in industrial applications, establishing a robust microbial platform for sustainable bioproduction.