Scholarlink

<p>Bacterial extracellular vesicles (BEVs) are nanosized, membrane-bound structures secreted by bacteria, playing diverse roles in intercellular communication, pathogenesis, stress tolerance, and immune modulation. Due to their ability to naturally transport biomolecules, BEVs are emerging as promising vehicles for drug delivery. This study explores the impact of protein cargo localization within BEVs on intracellular delivery efficiency, a critical factor in optimizing BEV-based therapeutics. We engineered <i>Escherichia coli</i> to express green fluorescent protein (GFP) in two distinct locations: the vesicular lumen (BEVs-cGFP) and the vesicular membrane (BEVs-mGFP). Following isolation, the BEVs were characterized for GFP localization and physicochemical properties. We then compared the intracellular transport efficiencies of BEVs-cGFP and BEVs-mGFP into macrophages (RAW&#xa0;264.7). Our results demonstrate that the localization of GFP within BEVs has a limited impact on their cellular uptake, suggesting that effective intracellular delivery can be achieved without strict control over cargo positioning. These findings provide valuable insights into the influence of protein cargo localization within BEVs on cellular uptake, highlighting the flexibility of BEVs as drug delivery vehicles. This study contributes to the broader field of nanomedicine, providing a framework for the development of advanced BEV platforms for cancer, neurodegenerative diseases, and other conditions.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Intracellular transport efficiencies of bacterial extracellular vesicles containing cytosolic and membrane-associated protein cargos

  • 期刊论文

  • 发表日期：

    2025年9月26日

查看全文
  • Ae-Ran Park,
  • Jong-Ha Park,
  • Sung In Lim

摘要

Bacterial extracellular vesicles (BEVs) are nanosized, membrane-bound structures secreted by bacteria, playing diverse roles in intercellular communication, pathogenesis, stress tolerance, and immune modulation. Due to their ability to naturally transport biomolecules, BEVs are emerging as promising vehicles for drug delivery. This study explores the impact of protein cargo localization within BEVs on intracellular delivery efficiency, a critical factor in optimizing BEV-based therapeutics. We engineered Escherichia coli to express green fluorescent protein (GFP) in two distinct locations: the vesicular lumen (BEVs-cGFP) and the vesicular membrane (BEVs-mGFP). Following isolation, the BEVs were characterized for GFP localization and physicochemical properties. We then compared the intracellular transport efficiencies of BEVs-cGFP and BEVs-mGFP into macrophages (RAW 264.7). Our results demonstrate that the localization of GFP within BEVs has a limited impact on their cellular uptake, suggesting that effective intracellular delivery can be achieved without strict control over cargo positioning. These findings provide valuable insights into the influence of protein cargo localization within BEVs on cellular uptake, highlighting the flexibility of BEVs as drug delivery vehicles. This study contributes to the broader field of nanomedicine, providing a framework for the development of advanced BEV platforms for cancer, neurodegenerative diseases, and other conditions.