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<p>This study reported mixotrophic bioplastics production by a mutant strain of <i>Cupriavidus</i> <i>necator</i> using molasses and palm oil as carbon sources. The mutant was developed through chemical mutagenesis coupled with fluorescence-based high-throughput screening resulting in a mutant strain MT-68 with enhanced polyhydroxyalkanoates (PHA) content by 78% compared to its parent. The strain was further optimized in pulse-feeding fed-batch process to enhance PHA titer to 8.2&#xa0;g/L and 47% content under mixotrophic fermentation of molasses and palm oil. Mixotrophic process also yielded scl-mcl copolymers of PHA composing of 3HB, 3HV, 3HHx and 3HO monomers. Bioreactor and shake flask operations also yielded similar PHA production, proving scalability of the optimized process. This study offered a promising low-cost bioplastics production through the mixotrophic conversion of molasses and waste palm oil by <i>C. necator</i>. The scl-mcl copolymers of PHA under mixotrophy could lead to production of bioplastics with various properties and applications.</p>

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Mixotrophic production of bioplastics from agricultural feedstocks by a mutant strain of Cupriavidus necator

  • 期刊论文

  • 发表日期：

    2025年8月14日

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  • Punpaporn Ketsakhon,
  • Napaporn Sittirangsinan,
  • Nareerat Yoriphan,
  • Pornkamol Unrean,
  • Verawat Champreda

摘要

This study reported mixotrophic bioplastics production by a mutant strain of Cupriavidus necator using molasses and palm oil as carbon sources. The mutant was developed through chemical mutagenesis coupled with fluorescence-based high-throughput screening resulting in a mutant strain MT-68 with enhanced polyhydroxyalkanoates (PHA) content by 78% compared to its parent. The strain was further optimized in pulse-feeding fed-batch process to enhance PHA titer to 8.2 g/L and 47% content under mixotrophic fermentation of molasses and palm oil. Mixotrophic process also yielded scl-mcl copolymers of PHA composing of 3HB, 3HV, 3HHx and 3HO monomers. Bioreactor and shake flask operations also yielded similar PHA production, proving scalability of the optimized process. This study offered a promising low-cost bioplastics production through the mixotrophic conversion of molasses and waste palm oil by C. necator. The scl-mcl copolymers of PHA under mixotrophy could lead to production of bioplastics with various properties and applications.