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<p>Furfural is primarily produced by pentose sugar dehydration during lignocellulose pretreatment. Breeding furfural-tolerant&#xa0;<i>Saccharomyces cerevisiae</i> is of great significance for the bioethanol production from biomass. In this study, comparative transcriptomics was employed to mine furfural tolerance-related genes in <i>S. cerevisiae</i>. Further, <i>S. cerevisiae</i> engineering strains were constructed to increase ethanol yield using corn stalk hydrolysate rich in furfural as a fermentation broth. The results showed that two <i>S. cerevisiae</i> genes of <i>pdb1</i> and <i>rts1</i> were screened with significant down-regulation under stress conditions of high-concentration furfural. Three engineering <i>S. cerevisiae</i> strains with <i>pdb1</i>, <i>rts1</i>, and <i>pdb1</i>/<i>rts1</i> deletion were constructed by the Clustered Regularly Interspaced Short Palindromic Repeats Cas9 technology. <i>S. cerevisiae pdb1Δrts1Δ</i> exhibited the highest furfural tolerance among the three engineered yeast strains. The level of lipid peroxidation and the content of reactive oxygen species of <i>S. cerevisiae pdb1Δrts1Δ</i> decreased by 17.2% and 18.7% compared to the wild-type strain, respectively. The ethanol yield of <i>S. cerevisiae pdb1Δrts1Δ</i> (26.32&#xa0;g/L) was 2.18 times compared to the wild-type strain (12.06&#xa0;g/L) in corn stalk hydrolysate. Therefore, <i>S. cerevisiae pdb1</i> and <i>rts1</i> associated with furfural tolerance would provide new gene materials for developing engineering yeasts for ethanol production using the lignocellulosic hydrolysate rich in furfural.</p>

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Gene mining of furfural tolerance-related S. cerevisiae pdb1 and rts1 and engineering yeast to increase ethanol yields using corn stalk hydrolysate

  • 期刊论文

  • 发表日期：

    2025年7月30日

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  • Peizhou Yang,
  • Yajun Tao,
  • Jianchao Chen,
  • Zhi Zheng

摘要

Furfural is primarily produced by pentose sugar dehydration during lignocellulose pretreatment. Breeding furfural-tolerant Saccharomyces cerevisiae is of great significance for the bioethanol production from biomass. In this study, comparative transcriptomics was employed to mine furfural tolerance-related genes in S. cerevisiae. Further, S. cerevisiae engineering strains were constructed to increase ethanol yield using corn stalk hydrolysate rich in furfural as a fermentation broth. The results showed that two S. cerevisiae genes of pdb1 and rts1 were screened with significant down-regulation under stress conditions of high-concentration furfural. Three engineering S. cerevisiae strains with pdb1, rts1, and pdb1/rts1 deletion were constructed by the Clustered Regularly Interspaced Short Palindromic Repeats Cas9 technology. S. cerevisiae pdb1Δrts1Δ exhibited the highest furfural tolerance among the three engineered yeast strains. The level of lipid peroxidation and the content of reactive oxygen species of S. cerevisiae pdb1Δrts1Δ decreased by 17.2% and 18.7% compared to the wild-type strain, respectively. The ethanol yield of S. cerevisiae pdb1Δrts1Δ (26.32 g/L) was 2.18 times compared to the wild-type strain (12.06 g/L) in corn stalk hydrolysate. Therefore, S. cerevisiae pdb1 and rts1 associated with furfural tolerance would provide new gene materials for developing engineering yeasts for ethanol production using the lignocellulosic hydrolysate rich in furfural.