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<p>The production of higher alcohols from renewable sources by microorganisms has been proposed to address the energy crisis and environmental concerns. A protein scaffold strategy was introduced in this study to physically co-localize the IlvA, Kivd and YqhD enzymes constituting the 1-propanol pathway. Through this strategy, the 1-propanol production became more efficient than in competing pathways. 3.8&#xa0;g/L of 1-propanol was produced at pH 7 and 37&#xa0;°C by the introduction of a protein scaffold without metabolic pathway engineering. By adjusting the ratio of the three enzymes, 4.5&#xa0;g/L of 1-propanol was produced with a recombinant strain containing the GDB:SH3:PDZ domains in a ratio of 1:2:1. These results suggest that the carbon flux was successfully directed to the 1-propanol pathway by employing protein scaffold strategy.</p>

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Engineering of Escherichia coli intracellular metabolism by introduction of protein scaffold for the efficient production of 1-propanol

  • 期刊论文

  • 发表日期：

    2025年7月1日

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  • Kim-Ngan T. Tran,
  • Jaehoon Jeong,
  • Jonguk Lee,
  • Soon Ho Hong

摘要

The production of higher alcohols from renewable sources by microorganisms has been proposed to address the energy crisis and environmental concerns. A protein scaffold strategy was introduced in this study to physically co-localize the IlvA, Kivd and YqhD enzymes constituting the 1-propanol pathway. Through this strategy, the 1-propanol production became more efficient than in competing pathways. 3.8 g/L of 1-propanol was produced at pH 7 and 37 °C by the introduction of a protein scaffold without metabolic pathway engineering. By adjusting the ratio of the three enzymes, 4.5 g/L of 1-propanol was produced with a recombinant strain containing the GDB:SH3:PDZ domains in a ratio of 1:2:1. These results suggest that the carbon flux was successfully directed to the 1-propanol pathway by employing protein scaffold strategy.