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<p>Hematopoietic stem cells (HSCs) derived from human umbilical cord blood (UCB) have gained significant attention for cell therapy applications due to their numerous advantages. With increasing research interest in the isolation and application of UCB-derived HSCs, establishing standardized protocols for their isolation and functional assessment is essential. While the process of isolating HSCs from UCB shares similarities with peripheral blood procedures, variations in experimental conditions, such as researcher skill and equipment, can impact reproducibility. In this study, we present a method that minimizes experimental variables, ensuring consistent results in UCB-derived HSC isolation. Additionally, we detail protocols to verify the functionality of isolated HSCs. These standardized methods will aid researchers in reliably isolating and characterizing UCB-derived HSCs for both experimental and therapeutic applications.</p>

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An efficient method for isolation and expansion of hematopoietic stem cells derived from human umbilical cord blood cells

  • 期刊论文

  • 发表日期：

    2025年4月18日

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  • Yo Seph Park,
  • Seokbeom Ham,
  • Hyunjoo Hwang,
  • Han Sung Hwang,
  • Kisung Ko,
  • Tae Jin Lee,
  • Kinarm Ko

摘要

Hematopoietic stem cells (HSCs) derived from human umbilical cord blood (UCB) have gained significant attention for cell therapy applications due to their numerous advantages. With increasing research interest in the isolation and application of UCB-derived HSCs, establishing standardized protocols for their isolation and functional assessment is essential. While the process of isolating HSCs from UCB shares similarities with peripheral blood procedures, variations in experimental conditions, such as researcher skill and equipment, can impact reproducibility. In this study, we present a method that minimizes experimental variables, ensuring consistent results in UCB-derived HSC isolation. Additionally, we detail protocols to verify the functionality of isolated HSCs. These standardized methods will aid researchers in reliably isolating and characterizing UCB-derived HSCs for both experimental and therapeutic applications.