Purpose <p>AGA is the most common cause of hair loss affecting female and male individuals at various ages. Separate topical treatments with minoxidil and Vitamin E (alpha-tocopherol) are available, but they are associated with various side effects and drawbacks, leading to poor patient compliance. The present work aims to develop a Polymeric microneedle patch for the co-delivery of minoxidil and Vitamin E.</p> Method <p>Various microneedle patch formulations (F1-F6) were prepared by the micro-mold casting method. The optimized formulation was selected based on scanning electron microscopy (SEM) analysis, Fourier transform infrared spectroscopy (FTIR), tensile strength, thickness, percentage elongation, in-vitro release study, ex-vivo permeation study, and in-vivo biocompatibility study.</p> Results <p>Based on the results, the F5 formulation containing a 2% thiolated chitosan solution was considered an optimised formulation, showing promising sustained-release properties (49.939 ± 5.92% for minoxidil and 51.513 ± 7.33% for Vitamin E). An improved permeation profile (40% drug permeation) was observed in the ex-vivo permeation study. Furthermore, irritation results (PII was 0.1 ± 1, showing negligible irritation, indicating its safety and biocompatibility to skin.</p> Conclusion <p>Thus, the optimized microneedle patch may show an enhanced hair growth effect through the synergistic effect of both minoxidil and Vitamin E.</p>