Scholarlink

Purpose <p>The unique qualities of metallic nanoparticles (1–100&#xa0;nm) have diverse biomedical applications. In this study, <i>Citrus maxima</i> (Cm) aqueous fruit peel extract was used to produce silver nanoparticles (AgNPs).</p> Methods <p>The synthesized Cm silver nanoparticles (CmAgNPs) has been studied for their nano characteristics using advanced microscopic evaluations and furthermore they were evaluated for its antibacterial, and antidiabetic applications. </p> Results <p>The colour changes from pale yellow to dark brown confirmed the formation of CmAgNPs. UV-Visible spectroscopy detected an absorption peak at 328 nm, and the stabilising functional groups were confirmed by Fourier-transform infrared (FTIR) spectroscopy. The zeta potential value was − 28.8 mV and the particle size was 172.23 nm, as confirmed through dynamic light scattering (DLS) analysis, while scanning electron spectroscopy (SEM) and transmission electron spectroscopy (TEM) confirmed that the nanoparticles were well-dispersed and spherically distributed (7–38 nm). The elemental nature of Ag was confirmed using Energy-dispersive X-ray spectroscopy (EDS). The CmAgNPs exhibited robust biological activity. The DPPH test confirmed the antioxidant activity of CmAgNPs, with an IC₅₀ of 26.4 µg/mL. Staphylococcus aureus, Pseudomonas aeruginosa, MRSA, and Escherichia coli exhibited good antibacterial activity, with inhibition zones as large as 18.5 mm against MRSA. Candida glabrata (22.67 mm) and Candida albicans (11 mm) were inhibited during the antifungal test. More than 75% viability of 3T3-L1 cells indicated biocompatibility of CmAgNPs, as determined using the MTT assay. Furthermore, CmAgNPs demonstrated good antidiabetic efficacy by improving glucose absorption (49.15%) and preventing lipid buildup (39%) in 3T3-L1 cells. Our study also confirmed the upregulation of GLUT4, PPAR-α, and adiponectin, whereas PPAR-γ and leptin genes were downregulated through RT-qPCR.</p> Conclusion <p>Our study showed that CmAgNPs possess multifunctional applications for contemporary health issues through their strong antioxidant, antibacterial, antidiabetic and antiadipogenic properties. However, further in vitro and in vivo studies on toxicity and molecular mechanism are recommended before using it for biomedical and pharmaceutical applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Bioengineered Citrus maxima mediated Silver Nanoparticles exhibit potent Antimicrobial, Anti-adipogenic and Antidiabetic effects

  • 期刊论文

  • 发表日期：

    2026年4月27日

查看全文
  • Shahnaz Majeed,
  • Nurul Shahirah Binti Nazri,
  • Ravindran Muthukumarasamy,
  • Mohammad Danish,
  • Sreenivas Patro Sisinthy,
  • Abdulaziz M. Alanazi,
  • Mohamad Nasir Bin Mohamad Ibrahim,
  • Mohammed Tahir Ansari,
  • Ohoud A. Jefri

摘要

Purpose

The unique qualities of metallic nanoparticles (1–100 nm) have diverse biomedical applications. In this study, Citrus maxima (Cm) aqueous fruit peel extract was used to produce silver nanoparticles (AgNPs).

Methods

The synthesized Cm silver nanoparticles (CmAgNPs) has been studied for their nano characteristics using advanced microscopic evaluations and furthermore they were evaluated for its antibacterial, and antidiabetic applications.

Results

The colour changes from pale yellow to dark brown confirmed the formation of CmAgNPs. UV-Visible spectroscopy detected an absorption peak at 328 nm, and the stabilising functional groups were confirmed by Fourier-transform infrared (FTIR) spectroscopy. The zeta potential value was − 28.8 mV and the particle size was 172.23 nm, as confirmed through dynamic light scattering (DLS) analysis, while scanning electron spectroscopy (SEM) and transmission electron spectroscopy (TEM) confirmed that the nanoparticles were well-dispersed and spherically distributed (7–38 nm). The elemental nature of Ag was confirmed using Energy-dispersive X-ray spectroscopy (EDS). The CmAgNPs exhibited robust biological activity. The DPPH test confirmed the antioxidant activity of CmAgNPs, with an IC₅₀ of 26.4 µg/mL. Staphylococcus aureus, Pseudomonas aeruginosa, MRSA, and Escherichia coli exhibited good antibacterial activity, with inhibition zones as large as 18.5 mm against MRSA. Candida glabrata (22.67 mm) and Candida albicans (11 mm) were inhibited during the antifungal test. More than 75% viability of 3T3-L1 cells indicated biocompatibility of CmAgNPs, as determined using the MTT assay. Furthermore, CmAgNPs demonstrated good antidiabetic efficacy by improving glucose absorption (49.15%) and preventing lipid buildup (39%) in 3T3-L1 cells. Our study also confirmed the upregulation of GLUT4, PPAR-α, and adiponectin, whereas PPAR-γ and leptin genes were downregulated through RT-qPCR.

Conclusion

Our study showed that CmAgNPs possess multifunctional applications for contemporary health issues through their strong antioxidant, antibacterial, antidiabetic and antiadipogenic properties. However, further in vitro and in vivo studies on toxicity and molecular mechanism are recommended before using it for biomedical and pharmaceutical applications.