Scholarlink

<p>Melanoma is a metastatic type of skin cancer with an increasing prevalence rate worldwide. <i>Lactobacillus rhamnosus</i> (<i>L. rhamnosus</i>) is a probiotic with valuable biological and pharmaceutical effects. To introduce a novel approach for melanoma treatment, we aimed to investigate the effect of <i>L. rhamnosus</i> on the growth and survival of mouse melanoma cells both in vitro and in vivo. B16-F10 and L929 cells were treated with serial dilutions of the heat-killed cell-free supernatant (CFS) of L. rhamnosus (1:10, 1:5, and 1:2.5) for four consecutive days. Cell proliferation was evaluated using alamarBlue and clonogenic assay. Flow cytometry was carried out using propidium iodide and annexin V–fluorescein isothiocyanate. For in vivo studies, B16-F10 cells were subcutaneously injected into C57BL/6 mice, and tumor volume, relative tumor volume, and tumor growth inhibition were calculated upon the injection of heat-killed CFS of L. rhamnosus. Findings indicated significant (p &lt; 0.001) reduction in the proliferation of B16-F10 cells upon treatment with the heat-killed CFS of L. rhamnosus, while no toxic effect was observed in normal fibroblasts. Results of clonogenic assay indicated significant (p &lt; 0.0001) decrease in the survival fraction of B16-F10 cells, and flow cytometry analysis revealed no noticeable apoptosis-inducing effects. Results of in vivo analysis indicated significant (p &lt; 0.01) decrease in tumor growth upon the injection of heat-killed CFS of L. rhamnosus into mouse melanoma models. In conclusion, the present study provides the first report that heat-killed CFS of L. rhamnosus inhibits B16-F10 melanoma cell growth in vitro and reduces tumor growth in vivo. To better evaluate observed therapeutic effects of this probiotic, more research on human melanoma cell lines is recommended.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Investigating the Effects of Heat-Killed Cell-Free Supernatant of Lactobacillus rhamnosus on Murine Melanoma Cells and Animal Models

  • 期刊论文

  • 发表日期：

    2026年4月15日

查看全文
  • Nafise Deldar,
  • Mehrdad Aghasizadeh,
  • Maryam A. Hosseinzadeh,
  • Maryam M. Matin,
  • Fatemeh B. Rassouli

摘要

Melanoma is a metastatic type of skin cancer with an increasing prevalence rate worldwide. Lactobacillus rhamnosus (L. rhamnosus) is a probiotic with valuable biological and pharmaceutical effects. To introduce a novel approach for melanoma treatment, we aimed to investigate the effect of L. rhamnosus on the growth and survival of mouse melanoma cells both in vitro and in vivo. B16-F10 and L929 cells were treated with serial dilutions of the heat-killed cell-free supernatant (CFS) of L. rhamnosus (1:10, 1:5, and 1:2.5) for four consecutive days. Cell proliferation was evaluated using alamarBlue and clonogenic assay. Flow cytometry was carried out using propidium iodide and annexin V–fluorescein isothiocyanate. For in vivo studies, B16-F10 cells were subcutaneously injected into C57BL/6 mice, and tumor volume, relative tumor volume, and tumor growth inhibition were calculated upon the injection of heat-killed CFS of L. rhamnosus. Findings indicated significant (p < 0.001) reduction in the proliferation of B16-F10 cells upon treatment with the heat-killed CFS of L. rhamnosus, while no toxic effect was observed in normal fibroblasts. Results of clonogenic assay indicated significant (p < 0.0001) decrease in the survival fraction of B16-F10 cells, and flow cytometry analysis revealed no noticeable apoptosis-inducing effects. Results of in vivo analysis indicated significant (p < 0.01) decrease in tumor growth upon the injection of heat-killed CFS of L. rhamnosus into mouse melanoma models. In conclusion, the present study provides the first report that heat-killed CFS of L. rhamnosus inhibits B16-F10 melanoma cell growth in vitro and reduces tumor growth in vivo. To better evaluate observed therapeutic effects of this probiotic, more research on human melanoma cell lines is recommended.