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Introduction <p>Chronic wounds represent a substantial clinical burden, underscoring the need for advanced therapeutic platforms capable of enhancing tissue regeneration and restoring skin integrity. In the present study, a novel hydrogel formulation incorporating <i>Coffea arabica</i> extract (1% w/w) and gentamicin sulphate (0.1% w/w) (CGSH) was developed and systematically evaluated. The performance of the developed formulation was comparatively assessed against a marketed collagen-based formulation containing gentamicin sulphate (0.1% w/w).</p> Methods <p>CGSH was formulated using <i>Coffea arabica</i> extract and Gentamicin Sulphate. The marketed comparator contained Gentamicin Sulphate (0.1% w/w) in a collagen base. Physicochemical parameters, including drug loading, batch quality, and transdermal permeation, were evaluated in vitro. The in vivo wound healing efficacy was assessed in Wistar rats through measurements of wound contraction and epithelialization.</p> Results <p>Formulation screening selection of formulation (F8) demonstrated significantly enhanced wound contraction (94.6 ± 2.1% on day 14) compared to marketed hydrogel (81.3 ± 3.4%, <i>p</i> &lt; 0.01), with a reduced epithelialization period (12.2 ± 0.8 days vs. 15.6 ± 1.1 days).</p> Discussion <p>The enhanced wound healing observed in this study is attributed to the synergistic effects of Gentamicin and the polyphenol-rich <i>Coffea arabica</i> extract, combining antibacterial activity with antioxidant-mediated modulation of inflammation and accelerated tissue regeneration. Unlike previous herbal Gentamicin hydrogels, the present formulation integrates a polyphenol-rich <i>Coffea arabica</i> extract with Gentamicin and provides comparative preclinical validation against a marketed formulation.</p> Graphical Abstract <p></p>

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Development and Preclinical Validation of a Novel Coffea arabica L. Extract-Infused Gentamicin Sulphate Hydrogel: Comparative Wound Healing Efficacy in a Rat

  • 期刊论文

  • 发表日期：

    2026年3月24日

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  • Roohi Yusufi,
  • Akanksha Dwivedi,
  • Gajanan N. Darwhekar,
  • Kuldeep Vinchurkar

摘要

Introduction

Chronic wounds represent a substantial clinical burden, underscoring the need for advanced therapeutic platforms capable of enhancing tissue regeneration and restoring skin integrity. In the present study, a novel hydrogel formulation incorporating Coffea arabica extract (1% w/w) and gentamicin sulphate (0.1% w/w) (CGSH) was developed and systematically evaluated. The performance of the developed formulation was comparatively assessed against a marketed collagen-based formulation containing gentamicin sulphate (0.1% w/w).

Methods

CGSH was formulated using Coffea arabica extract and Gentamicin Sulphate. The marketed comparator contained Gentamicin Sulphate (0.1% w/w) in a collagen base. Physicochemical parameters, including drug loading, batch quality, and transdermal permeation, were evaluated in vitro. The in vivo wound healing efficacy was assessed in Wistar rats through measurements of wound contraction and epithelialization.

Results

Formulation screening selection of formulation (F8) demonstrated significantly enhanced wound contraction (94.6 ± 2.1% on day 14) compared to marketed hydrogel (81.3 ± 3.4%, p < 0.01), with a reduced epithelialization period (12.2 ± 0.8 days vs. 15.6 ± 1.1 days).

Discussion

The enhanced wound healing observed in this study is attributed to the synergistic effects of Gentamicin and the polyphenol-rich Coffea arabica extract, combining antibacterial activity with antioxidant-mediated modulation of inflammation and accelerated tissue regeneration. Unlike previous herbal Gentamicin hydrogels, the present formulation integrates a polyphenol-rich Coffea arabica extract with Gentamicin and provides comparative preclinical validation against a marketed formulation.

Graphical Abstract