Scholarlink

Purpose <p>Iron nanoparticles are used in cancer treatment mainly as multifunctional platforms for magnetic hyperthermia, radiosensitization, drug delivery, and imaging-guided therapy, with several agents already in clinical use or in early trials. Their clinical role is still emerging, but they show particular promise in localized treatments (e.g., radiotherapy and hyperthermia enhancement) and image-guided interventions, with ongoing work on safety and immune effects. In this work, we described biogenically supported iron nanoparticles using leaves from <i>Lobelia erinus</i>.</p> Methods <p>The generated Fe NPs@<i>Lobelia erinus</i> were effectively stabilized by the leaf of <i>Lobelia erinus</i>. FE-SEM, FT-IR, XRD, TEM, EDX, and UV-Vis were used to physicochemically characterize the as-synthesized Fe NPs. The antioxidant effectiveness of the nanoparticles was evaluated using the DPPH method. The anticancer capabilities of biologically generated Fe NPs@<i>Lobelia </i><i>erinus</i> were assessed against breast cancer cells using the MTT assay, colony formation assay, molecular pathway analysis, and cell cycle flow cytometry.</p> Results <p>The Fe NPs’ antioxidant activity was noteworthy based on the IC<sub>50</sub> value. Recent nanoparticles appear to offer anti-human breast cancer potential due to their antioxidant properties. The anticancer properties of the Fe NPs@<i>Lobelia erinus</i> may successfully eradicate MCF7 cancer cells. Fe NPs@<i>Lobelia erinus</i> inhibit the formation of colonies, in contrast to their comparable control. Importantly, the molecular pathway analysis of cells exposed to Fe NPs@<i>Lobelia erinus</i> showed that the particles reduced the mRNA expression of p-STAT3/STAT3, Nrf2 and ARE in cells while increasing the expression of p53. This implies that the main mechanisms underlying the medical events that the extract induced in human breast cancer cells are STAT3 and p53. In cell cycle flow cytometry, the Fe NPs@<i>Lobelia erinus</i> considerably raised the proportion of the S phase and decreased the G0/G1 and G2/M phases percentages.</p> Conclusion <p>Biogenically synthesized Fe NPs@<i>Lobelia erinus</i> demonstrate significant anticancer potential against human breast cancer cells through STAT3 and p53 pathways.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Preparation, Chemical Characterization and Determination of the Antioxidant, Cytotoxicity and Anti-Breast Properties of Plant Extract Green-Synthesized Iron Nanoparticles

  • 期刊论文

  • 发表日期：

    2026年3月4日

查看全文
  • Weixun Lin,
  • Peng Du

摘要

Purpose

Iron nanoparticles are used in cancer treatment mainly as multifunctional platforms for magnetic hyperthermia, radiosensitization, drug delivery, and imaging-guided therapy, with several agents already in clinical use or in early trials. Their clinical role is still emerging, but they show particular promise in localized treatments (e.g., radiotherapy and hyperthermia enhancement) and image-guided interventions, with ongoing work on safety and immune effects. In this work, we described biogenically supported iron nanoparticles using leaves from Lobelia erinus.

Methods

The generated Fe NPs@Lobelia erinus were effectively stabilized by the leaf of Lobelia erinus. FE-SEM, FT-IR, XRD, TEM, EDX, and UV-Vis were used to physicochemically characterize the as-synthesized Fe NPs. The antioxidant effectiveness of the nanoparticles was evaluated using the DPPH method. The anticancer capabilities of biologically generated Fe NPs@Lobelia erinus were assessed against breast cancer cells using the MTT assay, colony formation assay, molecular pathway analysis, and cell cycle flow cytometry.

Results

The Fe NPs’ antioxidant activity was noteworthy based on the IC50 value. Recent nanoparticles appear to offer anti-human breast cancer potential due to their antioxidant properties. The anticancer properties of the Fe NPs@Lobelia erinus may successfully eradicate MCF7 cancer cells. Fe NPs@Lobelia erinus inhibit the formation of colonies, in contrast to their comparable control. Importantly, the molecular pathway analysis of cells exposed to Fe NPs@Lobelia erinus showed that the particles reduced the mRNA expression of p-STAT3/STAT3, Nrf2 and ARE in cells while increasing the expression of p53. This implies that the main mechanisms underlying the medical events that the extract induced in human breast cancer cells are STAT3 and p53. In cell cycle flow cytometry, the Fe NPs@Lobelia erinus considerably raised the proportion of the S phase and decreased the G0/G1 and G2/M phases percentages.

Conclusion

Biogenically synthesized Fe NPs@Lobelia erinus demonstrate significant anticancer potential against human breast cancer cells through STAT3 and p53 pathways.