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Purpose <p>Effective wound healing treatments are essential to prevent infections and in promoting recovery, as wounds can serve as entry points for various pathogens. This study aims to develop and characterize sodium hyaluronate based silver nanoparticle dispersion (SND) -loaded hydrogel for enhanced antimicrobial activity and improvedwound healing efficacy.</p> Method <p>Silver Nanoparticles (SNPs) were synthesized by the wet chemical method and stabilized with sodium hyaluronate, serving as a bifunctional excipient. The SNPs were characterized using UV-Visible spectroscopy, TEM (transmission electron microscopy), zeta sizer and ICP-AES (Inductively coupled plasma- atomic emission spectrometry). The optimized SNPs were then incorporated into a Carbopol<sup>®</sup> 974p based hydrogel matrix. The formulation was evaluated for in vitro drug release using the franz diffusion apparatus and then compared to a commercially available hydrogel, adhesive force, antimicrobial activity tested against Staphylococcus aureus and in vivo wound healing efficacy evaluated in rats.</p> Results <p>The optimized SNPs had an average size of 34 nm with a zeta potential of -13.7 mV, and ICP-AES analysis confirmed SNP concentration of approximately 51.91±1.52μg/ml. The developed hydrogel exhibited a controlled drug release pattern, with 23±0.89% drug diffusion and 77±1.25% retention, compared to 67±1.85% diffusion and 33±1.97% retention in the marketed formulation. Adhesive force was higher (± 1.05 g) than that of the conventional gel ( ± 0.87 g). The zone of inhibition against S. aureus was 23±1.2 mm for the test hydrogel versus 21±0.54 mm for the marketed product at 32ug/ml concentration. In vivo wound contraction reached ~90% by Day 16 with the SND loaded hydrogel (T52), significantly higher than ~75% in the marketed group and ~60% in the placebo. Histopathology confirmed enhanced angiogenesis and collagen formation in the treated group.</p> Conclusion <p>The sodium hyaluronate-stabilized SND loaded hydrogel exhibited exhibited superior adhesion, sustained drug release, potent antibacterial activity, and enhanced wound healing compared to conventional formulations. These findings suggest its potential as an effective therapeutic option for managing infected wounds and promoting tissue regeneration.</p> Graphical Abstract <p></p>

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Formulation and Evaluation of Sodium Hyaluronate- Based Silver Nanoparticle Hydrogel for Enhanced Antimicrobial Activity and Accelerated Wound Healing

  • 期刊论文

  • 发表日期：

    2026年3月10日

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  • Diksha kanojiya,
  • Sidra Khot,
  • Munira Momin

摘要

Purpose

Effective wound healing treatments are essential to prevent infections and in promoting recovery, as wounds can serve as entry points for various pathogens. This study aims to develop and characterize sodium hyaluronate based silver nanoparticle dispersion (SND) -loaded hydrogel for enhanced antimicrobial activity and improvedwound healing efficacy.

Method

Silver Nanoparticles (SNPs) were synthesized by the wet chemical method and stabilized with sodium hyaluronate, serving as a bifunctional excipient. The SNPs were characterized using UV-Visible spectroscopy, TEM (transmission electron microscopy), zeta sizer and ICP-AES (Inductively coupled plasma- atomic emission spectrometry). The optimized SNPs were then incorporated into a Carbopol® 974p based hydrogel matrix. The formulation was evaluated for in vitro drug release using the franz diffusion apparatus and then compared to a commercially available hydrogel, adhesive force, antimicrobial activity tested against Staphylococcus aureus and in vivo wound healing efficacy evaluated in rats.

Results

The optimized SNPs had an average size of 34 nm with a zeta potential of -13.7 mV, and ICP-AES analysis confirmed SNP concentration of approximately 51.91±1.52μg/ml. The developed hydrogel exhibited a controlled drug release pattern, with 23±0.89% drug diffusion and 77±1.25% retention, compared to 67±1.85% diffusion and 33±1.97% retention in the marketed formulation. Adhesive force was higher (± 1.05 g) than that of the conventional gel ( ± 0.87 g). The zone of inhibition against S. aureus was 23±1.2 mm for the test hydrogel versus 21±0.54 mm for the marketed product at 32ug/ml concentration. In vivo wound contraction reached ~90% by Day 16 with the SND loaded hydrogel (T52), significantly higher than ~75% in the marketed group and ~60% in the placebo. Histopathology confirmed enhanced angiogenesis and collagen formation in the treated group.

Conclusion

The sodium hyaluronate-stabilized SND loaded hydrogel exhibited exhibited superior adhesion, sustained drug release, potent antibacterial activity, and enhanced wound healing compared to conventional formulations. These findings suggest its potential as an effective therapeutic option for managing infected wounds and promoting tissue regeneration.

Graphical Abstract