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Background <p><i>Hibiscus rosa-sinensis </i>L, a widely used medicinal plant, has been traditionally recognized for its therapeutic properties, prompting investigations into its phytochemical composition and bioactivity.</p> Purpose <p>This study investigates the extraction and characterization of phytochemicals from <i>H. rosa-sinensis L.</i>, focusing on their potential antioxidant, antimitotic, and antiproliferative activities.</p> Methods <p>The extraction was conducted using various solvents by maceration method. Then different dilutions of extracts were used to carry out phytochemical analysis and bioactivities assessment.</p> Results <p>The yield analysis revealed that n-butanol produced the highest extraction yield (6.05%), while chloroform yielded the lowest (3.6%). Qualitative and quantitative assessments confirmed the presence of flavonoids, tannins, and phytosterols, with High-Performance Liquid Chromatography (HPLC) revealing Myricetin and Quercetin as the predominant flavonoids in the acetone extract, highlighting their potential health benefits due to their antioxidant properties. Furthermore, the antioxidant activity was highest in the acetone extract (71.13 ± 0.03%) at a concentration of 2&#xa0;mg/ml. The antimitotic activity was assessed using <i>Allium cepa</i> root tips, where the methanolic extract showed the strongest action, reducing the mitotic index significantly to 10% at 0.25&#xa0;mg/ml. The presence of chromosomal abnormalities, such as micronuclei and fragmented nuclei, confirmed the cytotoxic effects of the extracts. Additionally, the chloroform extract exhibited the lowest cell viability (25 ± 0.557%) in antiproliferative assays against yeast models, suggesting a significant cytotoxic potential.</p> Conclusion <p>The study concludes that <i>H. rosa-sinensis</i> possesses considerable antioxidant, antimitotic, and antiproliferative properties, warranting further exploration of its phytochemical constituents for potential therapeutic applications in drug discovery.</p>

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Determination of the Chemical Profile of Hibiscus rosa-sinensis L. Leaves Using Different Solvents and Evaluation of their Antioxidant, Antimitotic, and Antiproliferative Potentials

  • 期刊论文

  • 发表日期：

    2026年1月3日

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  • Sehrish Sadia,
  • Muhammad Waheed,
  • Tahira Rashid,
  • Murad Muhammad,
  • Huma Bashir,
  • Fahim Arshad,
  • Muhammad Iqbal,
  • Abeer Hashem,
  • Khalid F. Almutairi,
  • Elsayed Fathi Abd-Allah

摘要

Background

Hibiscus rosa-sinensis L, a widely used medicinal plant, has been traditionally recognized for its therapeutic properties, prompting investigations into its phytochemical composition and bioactivity.

Purpose

This study investigates the extraction and characterization of phytochemicals from H. rosa-sinensis L., focusing on their potential antioxidant, antimitotic, and antiproliferative activities.

Methods

The extraction was conducted using various solvents by maceration method. Then different dilutions of extracts were used to carry out phytochemical analysis and bioactivities assessment.

Results

The yield analysis revealed that n-butanol produced the highest extraction yield (6.05%), while chloroform yielded the lowest (3.6%). Qualitative and quantitative assessments confirmed the presence of flavonoids, tannins, and phytosterols, with High-Performance Liquid Chromatography (HPLC) revealing Myricetin and Quercetin as the predominant flavonoids in the acetone extract, highlighting their potential health benefits due to their antioxidant properties. Furthermore, the antioxidant activity was highest in the acetone extract (71.13 ± 0.03%) at a concentration of 2 mg/ml. The antimitotic activity was assessed using Allium cepa root tips, where the methanolic extract showed the strongest action, reducing the mitotic index significantly to 10% at 0.25 mg/ml. The presence of chromosomal abnormalities, such as micronuclei and fragmented nuclei, confirmed the cytotoxic effects of the extracts. Additionally, the chloroform extract exhibited the lowest cell viability (25 ± 0.557%) in antiproliferative assays against yeast models, suggesting a significant cytotoxic potential.

Conclusion

The study concludes that H. rosa-sinensis possesses considerable antioxidant, antimitotic, and antiproliferative properties, warranting further exploration of its phytochemical constituents for potential therapeutic applications in drug discovery.