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Purpose <p> This study introduces an innovative mixed-mode high-performance liquid chromatography (HPLC) method employing a Hi-Plex H column that synergistically combines size exclusion and ion exchange mechanisms, enabling precise and reproducible quantification of PVP K30 in diverse matrices, including pharmaceutical, cosmetic, and environmental water.</p> Methods <p> This study introduces an innovative mixed-mode high-performance liquid chromatography (HPLC) method employing a Hi-Plex H column using an isocratic 1:1 mix of 70% perchloric acid (pH ~1.5) and acetonitrile at 1.5 mL/min flow, 25°C, with detection at 210 nm and a 12-minute run time. A solid phase extraction pretreatment method was used to enhance their detection in wastewater. The method’s environmental impact was rigorously audited using established greenness metrics (AGREE, AGREEprep, MoGAPI, BAGI, and RGB12), providing a transparent assessment of its sustainability profile.</p> Results <p> The validated method demonstrated excellent linearity (4-500 μg/mL; r²= 0.9997) with limits of detection and quantification of 1.2 μg/mL and 3.8 μg/mL, respectively. Implementation of an optimized SPE protocol, providing a&#xa0;500-fold preconcentration, enabled exceptional environmental sensitivity (LOD: 2.4 ng/L in the original water sample). High accuracy was achieved with recovery rates of 98.35-99.45% in commercial products and 82.02-85.10% in water samples. A comprehensive evaluation using AGREE, AGREEprep, MoGAPI, BAGI, and RGB12 tools provided a multi-faceted perspective on the method's practicality and environmental footprint, with a high practical applicability score (BAGI = 70.0) and an overall whiteness score of 72.0.</p> Conclusion <p> The developed method represents a significant advancement in polymer analysis, providing a sensitive and versatile solution for PVP K30 monitoring in both industrial quality control and environmental protection applications.&#xa0;The integrated greenness assessment provides a benchmark for the environmental impact of this analysis and identifies areas for future improvement.&#xa0;</p> Graphical Abstract <p></p>

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A Versatile Mixed-Mode HPLC Method for Sensitive Quantification of Polyvinylpyrrolidone in Pharmaceutical, Cosmetic, and Environmental Samples with a Greenness Assessment

  • 期刊论文

  • 发表日期：

    2025年12月21日

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  • Samar M. Mahgoub,
  • Hassan A. Rudayni,
  • Abdelatty M. Radallada,
  • Heba A. Younes,
  • Ahmed Allam,
  • Rehab Mahmoud

摘要

Purpose

This study introduces an innovative mixed-mode high-performance liquid chromatography (HPLC) method employing a Hi-Plex H column that synergistically combines size exclusion and ion exchange mechanisms, enabling precise and reproducible quantification of PVP K30 in diverse matrices, including pharmaceutical, cosmetic, and environmental water.

Methods

This study introduces an innovative mixed-mode high-performance liquid chromatography (HPLC) method employing a Hi-Plex H column using an isocratic 1:1 mix of 70% perchloric acid (pH ~1.5) and acetonitrile at 1.5 mL/min flow, 25°C, with detection at 210 nm and a 12-minute run time. A solid phase extraction pretreatment method was used to enhance their detection in wastewater. The method’s environmental impact was rigorously audited using established greenness metrics (AGREE, AGREEprep, MoGAPI, BAGI, and RGB12), providing a transparent assessment of its sustainability profile.

Results

The validated method demonstrated excellent linearity (4-500 μg/mL; r²= 0.9997) with limits of detection and quantification of 1.2 μg/mL and 3.8 μg/mL, respectively. Implementation of an optimized SPE protocol, providing a 500-fold preconcentration, enabled exceptional environmental sensitivity (LOD: 2.4 ng/L in the original water sample). High accuracy was achieved with recovery rates of 98.35-99.45% in commercial products and 82.02-85.10% in water samples. A comprehensive evaluation using AGREE, AGREEprep, MoGAPI, BAGI, and RGB12 tools provided a multi-faceted perspective on the method's practicality and environmental footprint, with a high practical applicability score (BAGI = 70.0) and an overall whiteness score of 72.0.

Conclusion

The developed method represents a significant advancement in polymer analysis, providing a sensitive and versatile solution for PVP K30 monitoring in both industrial quality control and environmental protection applications. The integrated greenness assessment provides a benchmark for the environmental impact of this analysis and identifies areas for future improvement. 

Graphical Abstract