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Purpose <p>Chronic inflammation, a persistent immune response, contributes to tissue damage and the progression of diseases such as arthritis, diabetes, and cancer. Biocompatible, multifunctional nanomedicines offer a sustainable therapeutic approach. This study reports the green synthesis of silver nanoparticles (LM-AgNPs) using <i>Lantana montevidensis</i> leaf extract (LMLE) and their evaluation for multifunctional biological activities.</p> Methods <p>LM-AgNPs were synthesized via biogenic reduction of silver nitrate using LMLE, with optimization achieved by varying extract concentration, silver nitrate concentration, temperature, pH, and time. Characterization was performed using UV-Vis, SEM-EDX, TEM, FTIR, and XRD analyses. Biological activities were assessed through antioxidant (DPPH, HSPA, FRAP), anti-inflammatory (protein denaturation, membrane stabilization), antidiabetic (α-amylase, α-glucosidase inhibition), cytotoxicity (MCF-7, L929 cells) and in vivo anti-arthritic assays in CFA-induced arthritic rats.</p> Results <p>LM-AgNPs were confirmed as spherical, crystalline nanoparticles with a mean size of 98.64&#xa0;nm and zeta potential of -27 mV. LM-AgNPs exhibited strong antioxidant activity (FRAP 49.76 ± 0.18&#xa0;µg/mL; DPPH 28.88 ± 0.23&#xa0;µg/mL; HSPA 41.59 ± 0.23) as compared to ascorbic acid, significant anti-inflammatory effect (albumin inhibition 94.24 ± 1.90%) compared to aspirin, and antidiabetic activity (α-amylase: 43.14 ± 0.86&#xa0;µg/mL; α-glucosidase: 59.41 ± 0.06&#xa0;µg/mL). Cytotoxicity assays revealed selective activity against MCF-7 breast cancer cells (IC<sub>50</sub> = 52.15&#xa0;µg/mL) while maintaining cell viability in normal fibroblasts (89.5–79.25%) comparable to 5-fluorouracil. In vivo, LM-AgNPs inhibited carrageenan-induced paw edema (92.95% at 6&#xa0;h) and demonstrated potent anti-arthritic activity (97.26%) compared to standard (95.89%), with reductions in TNF-α (62.91 ± 0.01 pg/mL) and IL-6 (136 ± 3 pg/mL). Radiographic and histopathological analyses confirmed joint protection.</p> Conclusion <p><i>L. montevidensis</i>-mediated silver nanoparticles exhibited multifunctional bioactivity, with nanoparticle formulation enhancing efficacy compared to the crude extract. These findings highlight their potential for translational applications in chronic, inflammation-driven diseases.</p> Graphical Abstract <p></p>

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Biogenic Synthesis and Optimization of Lantana Montevidensis-Mediated Silver Nanoparticles as a Multifunctional Therapeutic: Anti-Inflammatory and Cytotoxicity Evaluation

  • 期刊论文

  • 发表日期：

    2025年11月17日

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  • Sourabh Malabade,
  • Preeti Salve,
  • Pranay Shirke,
  • N. A Khatib

摘要

Purpose

Chronic inflammation, a persistent immune response, contributes to tissue damage and the progression of diseases such as arthritis, diabetes, and cancer. Biocompatible, multifunctional nanomedicines offer a sustainable therapeutic approach. This study reports the green synthesis of silver nanoparticles (LM-AgNPs) using Lantana montevidensis leaf extract (LMLE) and their evaluation for multifunctional biological activities.

Methods

LM-AgNPs were synthesized via biogenic reduction of silver nitrate using LMLE, with optimization achieved by varying extract concentration, silver nitrate concentration, temperature, pH, and time. Characterization was performed using UV-Vis, SEM-EDX, TEM, FTIR, and XRD analyses. Biological activities were assessed through antioxidant (DPPH, HSPA, FRAP), anti-inflammatory (protein denaturation, membrane stabilization), antidiabetic (α-amylase, α-glucosidase inhibition), cytotoxicity (MCF-7, L929 cells) and in vivo anti-arthritic assays in CFA-induced arthritic rats.

Results

LM-AgNPs were confirmed as spherical, crystalline nanoparticles with a mean size of 98.64 nm and zeta potential of -27 mV. LM-AgNPs exhibited strong antioxidant activity (FRAP 49.76 ± 0.18 µg/mL; DPPH 28.88 ± 0.23 µg/mL; HSPA 41.59 ± 0.23) as compared to ascorbic acid, significant anti-inflammatory effect (albumin inhibition 94.24 ± 1.90%) compared to aspirin, and antidiabetic activity (α-amylase: 43.14 ± 0.86 µg/mL; α-glucosidase: 59.41 ± 0.06 µg/mL). Cytotoxicity assays revealed selective activity against MCF-7 breast cancer cells (IC50 = 52.15 µg/mL) while maintaining cell viability in normal fibroblasts (89.5–79.25%) comparable to 5-fluorouracil. In vivo, LM-AgNPs inhibited carrageenan-induced paw edema (92.95% at 6 h) and demonstrated potent anti-arthritic activity (97.26%) compared to standard (95.89%), with reductions in TNF-α (62.91 ± 0.01 pg/mL) and IL-6 (136 ± 3 pg/mL). Radiographic and histopathological analyses confirmed joint protection.

Conclusion

L. montevidensis-mediated silver nanoparticles exhibited multifunctional bioactivity, with nanoparticle formulation enhancing efficacy compared to the crude extract. These findings highlight their potential for translational applications in chronic, inflammation-driven diseases.

Graphical Abstract