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Background <p>Burn injuries remain a significant global health concern. Apricots (<i>Prunus armeniaca</i>), which belong to the Rosaceae family, are temperate fruits rich in carotenoids, phenolic compounds, and flavonoids. These bioactive constituents enhance the fruit’s colour, taste, and nutritional value, while also offering antioxidant and disease-preventive benefits that support overall health.</p> Objective <p>To formulate and optimize an apricot-loaded phytosomal gel and evaluate its wound healing efficacy in a rat burn wound model.</p> Methods <p>Apricot-loaded phytosomes were formulated by the thin-film hydration method and optimized using a Box-Behnken design, with phospholipid concentration, temperature, and rotation speed as independent variables. Characterization included vesicle size, entrapment efficiency, vesicle morphology, and gel properties. In vivo studies involved second-degree burn wounds in Wistar rats, divided into control, SSD-treated, and apricot phytosomal gel-treated groups. Wound healing efficacy was evaluated through %wound contraction, epithelialization, biochemical markers (hydroxyproline, hexosamine), oxidative stress markers (SOD, GSH, MDA), and histopathological analysis of wound tissue.</p> Results <p>The optimized formulation showed vesicle size 101.7&#xa0;nm, entrapment efficiency 78.6%, uniform spherical shape, acceptable gel properties, higher in-vitro diffusion and ex-vivo permeation studies. In vivo, the phytosomal gel achieved 99% wound contraction by day 20, significantly higher than the control and comparable to SSD. Biochemical analyses revealed increased hydroxyproline and hexosamine, indicating enhanced collagen synthesis. Oxidative stress markers improved, and histopathology confirmed enhanced re-epithelialization, angiogenesis, and reduced inflammation.</p> Conclusion <p>Apricot-loaded phytosomal gel demonstrated significant burn wound healing efficacy, supported by antioxidant activity and collagen regeneration, suggesting it as a promising natural alternative for burn treatment.</p> Graphical Abstract <p></p>

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Exploring the Therapeutic Potential of Apricot-Loaded Phytosomal Gel for Burn Wound Healing in Wistar Rats

  • 期刊论文

  • 发表日期：

    2025年10月17日

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  • Manisha Rajpurohit,
  • Mrityunjaya Patil,
  • Nayeem Khatib

摘要

Background

Burn injuries remain a significant global health concern. Apricots (Prunus armeniaca), which belong to the Rosaceae family, are temperate fruits rich in carotenoids, phenolic compounds, and flavonoids. These bioactive constituents enhance the fruit’s colour, taste, and nutritional value, while also offering antioxidant and disease-preventive benefits that support overall health.

Objective

To formulate and optimize an apricot-loaded phytosomal gel and evaluate its wound healing efficacy in a rat burn wound model.

Methods

Apricot-loaded phytosomes were formulated by the thin-film hydration method and optimized using a Box-Behnken design, with phospholipid concentration, temperature, and rotation speed as independent variables. Characterization included vesicle size, entrapment efficiency, vesicle morphology, and gel properties. In vivo studies involved second-degree burn wounds in Wistar rats, divided into control, SSD-treated, and apricot phytosomal gel-treated groups. Wound healing efficacy was evaluated through %wound contraction, epithelialization, biochemical markers (hydroxyproline, hexosamine), oxidative stress markers (SOD, GSH, MDA), and histopathological analysis of wound tissue.

Results

The optimized formulation showed vesicle size 101.7 nm, entrapment efficiency 78.6%, uniform spherical shape, acceptable gel properties, higher in-vitro diffusion and ex-vivo permeation studies. In vivo, the phytosomal gel achieved 99% wound contraction by day 20, significantly higher than the control and comparable to SSD. Biochemical analyses revealed increased hydroxyproline and hexosamine, indicating enhanced collagen synthesis. Oxidative stress markers improved, and histopathology confirmed enhanced re-epithelialization, angiogenesis, and reduced inflammation.

Conclusion

Apricot-loaded phytosomal gel demonstrated significant burn wound healing efficacy, supported by antioxidant activity and collagen regeneration, suggesting it as a promising natural alternative for burn treatment.

Graphical Abstract