Scholarlink

Objective(s) <p>Recently, combination therapy for cancer has been shown to be more effective than monotherapy, resulting in reduced side effects and increased treatment efficacy. Melatonin, a naturally produced hormone, exhibits anticancer properties by inducing apoptosis and modulating the immune system; however, its short half-life and variable bioavailability limit its effectiveness. The current research examines how melatonin-chitosan-phosphatidylcholine nanoparticles (Cht-Pc/MLT NPs), as a novel lipid-polymer nanosystem, could enhance the anticancer properties of doxorubicin (DOX) against colorectal cancer cell lines (C26).</p> Methods <p>The Cht-Pc/MLT NPs were synthesized using the self-assembly method. The formulations’ physicochemical characterizations, including particle size, stability, and release profile, were investigated. The cytotoxicity evaluation was conducted independently and with free DOX on the C26 cell line using the MTT and sub-G1 assays.</p> Results <p>The optimal formulation displayed a size of 173 ± 18.17&#xa0;nm, a surface charge of 16.28 mV, encapsulation efficiency and loading capacity of 63.148% and 30.861%, respectively. It was observed that the release of MLT from the NPs in simulated gastric fluid (SGF, pH 1.2) was minimal during the initial 4&#xa0;h, making it suitable for oral administration. On the other hand, in intestinal fluid (SIF, pH 6.8), about 30% of MLT was released within the first 4&#xa0;h, and at the end of 48&#xa0;h, the release reached 45%. The observed changes in particle size after three months of storage at 4&#xa0;°C were negligible obtained. Our research demonstrated that the combined delivery of DOX and Cht-Pc/MLT NPs significantly enhanced the cytotoxicity of colorectal cancer cells (C26) compared to other groups.</p> Conclusion <p>The results indicate that this designed combination treatment system may be applicable in chemotherapy protocols.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Enhancing Doxorubicin Efficacy with a Novel Lipid-Polymer Nanosystem Containing Melatonin: Formulation, Characterization and in Vitro Study

  • 期刊论文

  • 发表日期：

    2025年9月18日

查看全文
  • Somayeh Marouzi,
  • Zahra Boroghani,
  • Ghasem Anbiaee,
  • Vahid Fattahi Moghaddam,
  • Zahra Salmasi,
  • Fatemeh Kalalinia,
  • Maryam Hashemi

摘要

Objective(s)

Recently, combination therapy for cancer has been shown to be more effective than monotherapy, resulting in reduced side effects and increased treatment efficacy. Melatonin, a naturally produced hormone, exhibits anticancer properties by inducing apoptosis and modulating the immune system; however, its short half-life and variable bioavailability limit its effectiveness. The current research examines how melatonin-chitosan-phosphatidylcholine nanoparticles (Cht-Pc/MLT NPs), as a novel lipid-polymer nanosystem, could enhance the anticancer properties of doxorubicin (DOX) against colorectal cancer cell lines (C26).

Methods

The Cht-Pc/MLT NPs were synthesized using the self-assembly method. The formulations’ physicochemical characterizations, including particle size, stability, and release profile, were investigated. The cytotoxicity evaluation was conducted independently and with free DOX on the C26 cell line using the MTT and sub-G1 assays.

Results

The optimal formulation displayed a size of 173 ± 18.17 nm, a surface charge of 16.28 mV, encapsulation efficiency and loading capacity of 63.148% and 30.861%, respectively. It was observed that the release of MLT from the NPs in simulated gastric fluid (SGF, pH 1.2) was minimal during the initial 4 h, making it suitable for oral administration. On the other hand, in intestinal fluid (SIF, pH 6.8), about 30% of MLT was released within the first 4 h, and at the end of 48 h, the release reached 45%. The observed changes in particle size after three months of storage at 4 °C were negligible obtained. Our research demonstrated that the combined delivery of DOX and Cht-Pc/MLT NPs significantly enhanced the cytotoxicity of colorectal cancer cells (C26) compared to other groups.

Conclusion

The results indicate that this designed combination treatment system may be applicable in chemotherapy protocols.