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Purpose <p>This study aimed to evaluate the wound healing potential of green-synthesized zinc oxide nanoparticles (ZnO NPs) using <i>Moringa oleifera</i> leaf extract as an alternative material for wound dressings, leveraging their biocompatibility and physicochemical properties.</p> Methods <p>Methanolic extract of <i>M. oleifera</i> leaves was analyzed using LC-MS/MS to identify bioactive compounds. ZnO NPs were synthesized using the extract and characterized via XRD, FTIR, and SEM. Proximate analysis determined moisture content, while antioxidant and antibacterial activities were assessed. In <i>vivo</i> studies evaluated wound contraction, tissue growth, and histopathological changes.</p> Results <p>LC-MS/MS analysis confirmed the presence of bioactive compounds in the methanolic extract, which are known to aid wound healing. The synthesized ZnO NPs exhibited a hexagonal shape with an average particle size of 19.2&#xa0;nm. XRD analytical observations and FTIR analysis confirmed the stabilization of ZnONPs. The ZnO NPs demonstrated strong antioxidant activity (68.99% scavenging potential) and superior antibacterial activity (zone of inhibition: 19.3 ± 1.1&#xa0;mm) compared to the methanolic extract. They also showed higher phenolic (47.11 ± 2.0&#xa0;mg GAE/g DW) and flavonoid (3.39 ± 0.62&#xa0;mg QE/g DW) content. In vivo studies revealed that ZnO NPs significantly promoted wound contraction, tissue regeneration, and collagen deposition while reducing inflammation. Histopathological analysis further confirmed enhanced epithelial regeneration and angiogenesis in wounds treated with ZnO NPs.</p> Conclusion <p>Green-synthesized ZnO NPs using <i>M. oleifera</i> extract show excellent potential as wound dressing materials due to their antioxidant, antibacterial, and wound-healing properties, promoting tissue regeneration and reducing inflammation effectively.</p>

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Green-Synthesized ZnO Nanoparticles Using Methanolic Extract of Moringa oleifera Leaves: Characterization and Evaluation of Wound Healing Efficacy

  • 期刊论文

  • 发表日期：

    2025年6月16日

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  • Mazhar Abbas,
  • Aqsa Mumtaz,
  • Osama A. Mohammed,
  • Kinza Zafar,
  • Ishtiaq Ahmed,
  • Muhammad T. Iqbal,
  • Munawar Iqbal,
  • Arif Nazir,
  • Ali M. S. Eleragi,
  • Lobna A. Saleh,
  • Mohammed A. Attia,
  • Emad Bahashwan,
  • AbdulElah A. J. AlQahtani,
  • Sameer M. Khan,
  • Walaa A. El-Dakroury,
  • Ahmed S. Doghish

摘要

Purpose

This study aimed to evaluate the wound healing potential of green-synthesized zinc oxide nanoparticles (ZnO NPs) using Moringa oleifera leaf extract as an alternative material for wound dressings, leveraging their biocompatibility and physicochemical properties.

Methods

Methanolic extract of M. oleifera leaves was analyzed using LC-MS/MS to identify bioactive compounds. ZnO NPs were synthesized using the extract and characterized via XRD, FTIR, and SEM. Proximate analysis determined moisture content, while antioxidant and antibacterial activities were assessed. In vivo studies evaluated wound contraction, tissue growth, and histopathological changes.

Results

LC-MS/MS analysis confirmed the presence of bioactive compounds in the methanolic extract, which are known to aid wound healing. The synthesized ZnO NPs exhibited a hexagonal shape with an average particle size of 19.2 nm. XRD analytical observations and FTIR analysis confirmed the stabilization of ZnONPs. The ZnO NPs demonstrated strong antioxidant activity (68.99% scavenging potential) and superior antibacterial activity (zone of inhibition: 19.3 ± 1.1 mm) compared to the methanolic extract. They also showed higher phenolic (47.11 ± 2.0 mg GAE/g DW) and flavonoid (3.39 ± 0.62 mg QE/g DW) content. In vivo studies revealed that ZnO NPs significantly promoted wound contraction, tissue regeneration, and collagen deposition while reducing inflammation. Histopathological analysis further confirmed enhanced epithelial regeneration and angiogenesis in wounds treated with ZnO NPs.

Conclusion

Green-synthesized ZnO NPs using M. oleifera extract show excellent potential as wound dressing materials due to their antioxidant, antibacterial, and wound-healing properties, promoting tissue regeneration and reducing inflammation effectively.