Purpose <p>This study aimed to formulate and evaluate a herbal lip balm for the treatment of cheilitis. Angular cheilitis presents as a roughly triangular area of erythema and edema at one or, more commonly, both corners of the mouth. The most frequent cause of angular cheilitis in adults is a fungal infection caused by <i>Candida albicans,</i> and less frequently, <i>Staphylococcus aureus</i>.</p> Methods <p>Plant extracts were obtained through Soxhlet extraction, and the concentration for the final formulation was optimized using Design of Experiments (DOE). Organoleptic evaluation and antimicrobial studies were conducted. In vitro cytotoxicity studies were conducted using MTT assay. Skin irritancy was assessed using the HET-CAM test, while the wound healing potential was confirmed through a scratch wound healing assay.</p> Results <p>The formulated lip balm exhibited a pleasant appearance, uniform distribution, and was free from grittiness. Antimicrobial studies demonstrated significant antibacterial activity and promising antifungal properties against angular cheilitis-causing pathogens. The HET-CAM test confirmed that the lip balm was free from skin irritancy. Safety was further verified through in vitro cytotoxicity testing via the MTT assay, with an LC50 value of 299.957&#xa0;µg/ml. High cell viability, even at 100&#xa0;µg/ml, indicating no toxicity in cultured cell lines. The Scratch assay confirmed the lip balm's wound healing potential, reinforcing the formulation's efficacy.</p> Conclusion <p>The formulation was found to be safe and effective, suggesting its potential as a therapeutic option for angular cheilitis. This study offers a systematic approach to developing herbal lip balm using natural extracts known for their medicinal properties<b>.</b></p>