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<p>To broaden genetic diversity in the breeders’ gene pool, we previously selected 95 long-day-adapted hybrid clones from Andean cultivated potatoes. In this study, these clones were crossed to combine resistance genes for golden cyst nematode (<i>H1</i>), <i>Potato virus Y</i> (<i>Ry</i><sub><i>chc</i></sub>), and <i>Potato virus X</i> (<i>Rx1</i>) into individual clones using marker assays through multiplex PCR. Additionally, effective late blight resistance genes, <i>Rpi-blb3</i> and/or <i>R2</i>, were incorporated. After field evaluation, the selected clones were crossed and marker-assayed with real-time PCR to increase the copy number (dosage) of the resistance genes. After two rounds of increasing the copy number, 16 clones exhibited an average copy number of 3.00 for <i>H1</i>, 2.56 for <i>Ry</i><sub><i>chc</i></sub>, and 2.81 for <i>Rx1</i>, while 18 clones showed an average copy number of 2.56 for <i>H1</i>, 1.94 for <i>Ry</i><sub><i>chc</i></sub>, and 2.06 for <i>Rx1,</i> along with 0.83 for <i>Rpi-blb3</i> and 0.61 for <i>R2</i>. These 34 clones possess multiple, multiplexed resistance genes, which are valuable for breeding programs.</p>

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Creation of Clones with Multiple Multiplexed Resistance Genes Useful in Potato Breeding

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月23日

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  • Kazuyoshi Hosaka,
  • Rena Sanetomo,
  • Kenji Katayama,
  • Seiji Tamiya,
  • Kenji Asano,
  • Etsuo Shimosaka,
  • Kotaro Akai,
  • Hitomi Nakajima,
  • Satoshi Okamoto

摘要

To broaden genetic diversity in the breeders’ gene pool, we previously selected 95 long-day-adapted hybrid clones from Andean cultivated potatoes. In this study, these clones were crossed to combine resistance genes for golden cyst nematode (H1), Potato virus Y (Rychc), and Potato virus X (Rx1) into individual clones using marker assays through multiplex PCR. Additionally, effective late blight resistance genes, Rpi-blb3 and/or R2, were incorporated. After field evaluation, the selected clones were crossed and marker-assayed with real-time PCR to increase the copy number (dosage) of the resistance genes. After two rounds of increasing the copy number, 16 clones exhibited an average copy number of 3.00 for H1, 2.56 for Rychc, and 2.81 for Rx1, while 18 clones showed an average copy number of 2.56 for H1, 1.94 for Rychc, and 2.06 for Rx1, along with 0.83 for Rpi-blb3 and 0.61 for R2. These 34 clones possess multiple, multiplexed resistance genes, which are valuable for breeding programs.