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<p><i>Solanum fendleri</i> (fen) is one of two wild potato species native to the USA. The US Potato Genebank has 269 samples, over 90% of which were collected by the authors in annual expeditions to the states of Arizona, New Mexico, and Texas since 1992. For the ultimate in efficiency for germplasm representation of a species in the genebank one would: (1) acquire many population samples from the broadest possible eco-geographic representation of the range, (2) identify the minimum number of populations that collectively capture the maximum amount of diversity (a core subset), (3) identify the minimum number of individuals which, when combined as parents, collectively capture the maximum genetic diversity in a single new synthetic population. In 2016 we published a recommended core subset of fen from the USA. In 2020 we reported the discovery of a single very large “mega-population” of fen near Tucson, AZ that by itself could be considered a core subset of the species, capturing most of the known USA fen genetic diversity. We further optimized sampling of that mega-population site with more intensive sampling as 160 dispersed individuals, genotyping them at 32&#xa0;K GBS loci, removing redundant individuals and selecting only 38 plants that capture 93% of the allele diversity and 71% of unique alleles, and then intermating those 38 plants. This study is presented as a model for composing the ultimate genebank sample of a species– the richest and most efficient representation of fen diversity in the USA packed into a single seedlot for sharing with researchers, breeders, and other genebanks.</p>

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An Optimized Core Sample of the Wild Potato Solanum fendleri in the USA

  • 期刊论文

  • 发表日期：

    2025年9月3日

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  • John Bamberg,
  • Ingrid Bamberg,
  • Alfonso del Rio,
  • Chico Fernandez

摘要

Solanum fendleri (fen) is one of two wild potato species native to the USA. The US Potato Genebank has 269 samples, over 90% of which were collected by the authors in annual expeditions to the states of Arizona, New Mexico, and Texas since 1992. For the ultimate in efficiency for germplasm representation of a species in the genebank one would: (1) acquire many population samples from the broadest possible eco-geographic representation of the range, (2) identify the minimum number of populations that collectively capture the maximum amount of diversity (a core subset), (3) identify the minimum number of individuals which, when combined as parents, collectively capture the maximum genetic diversity in a single new synthetic population. In 2016 we published a recommended core subset of fen from the USA. In 2020 we reported the discovery of a single very large “mega-population” of fen near Tucson, AZ that by itself could be considered a core subset of the species, capturing most of the known USA fen genetic diversity. We further optimized sampling of that mega-population site with more intensive sampling as 160 dispersed individuals, genotyping them at 32 K GBS loci, removing redundant individuals and selecting only 38 plants that capture 93% of the allele diversity and 71% of unique alleles, and then intermating those 38 plants. This study is presented as a model for composing the ultimate genebank sample of a species– the richest and most efficient representation of fen diversity in the USA packed into a single seedlot for sharing with researchers, breeders, and other genebanks.