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<p>The <i>Rx1</i> and <i>Rx2</i> genes from wild potato species confer extreme resistance to potato virus X (PVX). Accurate genotyping is essential for their integration into breeding programs. We developed tetra-primer ARMS-PCR markers targeting SNPs (G2515C in <i>Rx1</i> and C2445G in <i>Rx2</i>) in the LRR regions to distinguish resistance alleles. These markers effectively genotyped 50 tetraploid potato varieties, identifying 16 as <i>Rx1</i> heterozygous, 5 as <i>Rx2</i> heterozygous, and 29 as homozygous susceptible consistent with phenotypic data. The T-ARMS-PCR outperformed existing markers and enabled efficient duplex detection of both genes. Although no homozygous resistant varieties were found, artificial DNA mixtures validated marker reliability for homozygous and heterozygous resistance detection. To our knowledge, this is the first use of T-ARMS-PCR for <i>Rx1</i>/<i>Rx2</i> genotyping, offering a high-throughput tool for marker-assisted selection in developing PVX-resistant cultivars.</p>

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Precision SNP Genotyping for PVX Resistance Breeding in Potato: A Duplex Tetra-primer ARMS‒PCR Approach Targeting the Rx1 and Rx2 Genes

  • 期刊论文

  • 发表日期：

    2025年8月7日

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  • Saiful Islam,
  • Jiana Li,
  • Mohammad Ataur Rahman,
  • Jiling Song,
  • Binyan Xu,
  • Jie Zheng,
  • Siyu Zeng,
  • Botao Song,
  • Bihua Nie

摘要

The Rx1 and Rx2 genes from wild potato species confer extreme resistance to potato virus X (PVX). Accurate genotyping is essential for their integration into breeding programs. We developed tetra-primer ARMS-PCR markers targeting SNPs (G2515C in Rx1 and C2445G in Rx2) in the LRR regions to distinguish resistance alleles. These markers effectively genotyped 50 tetraploid potato varieties, identifying 16 as Rx1 heterozygous, 5 as Rx2 heterozygous, and 29 as homozygous susceptible consistent with phenotypic data. The T-ARMS-PCR outperformed existing markers and enabled efficient duplex detection of both genes. Although no homozygous resistant varieties were found, artificial DNA mixtures validated marker reliability for homozygous and heterozygous resistance detection. To our knowledge, this is the first use of T-ARMS-PCR for Rx1/Rx2 genotyping, offering a high-throughput tool for marker-assisted selection in developing PVX-resistant cultivars.