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<p>This study aimed to determine the optimal cultivation conditions for four <i>Escherichia coli</i> strains producing recombinant glucagon-like peptide-1 tandems. Validation experiments confirmed that scale-up from shake flasks to a bioreactor is feasible without a significant loss in specific productivity. We also demonstrated that the optimal cultivation conditions were identical for all tested strains. However, the number of glucagon-like peptide-1 monomers in the tandem affected the final yield in both shake flasks and the bioreactor. We also evaluated the target protein fraction in the wet inclusion bodies obtained. The amount of inclusion bodies per gram of biomass was found to correlate with specific productivity, while the target protein fraction in wet inclusion bodies varied among the different tandems. A correlation was identified between the titer of the glucagon-like peptide-1 tandems and the number of monomers. The results indicate that selecting an appropriate number of monomers during strain development can enhance productivity, improve the target protein fraction in wet inclusion bodies, and increase the final product titer after downstream processing.</p>

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The effect of monomers number in peptides on Escherichia coli strain productivity and inclusion bodies-associated target protein accumulation

  • 期刊论文

  • 发表日期：

    2026年6月19日

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  • I. A. Kornakov,
  • T. V. Mukhina,
  • E. A. Buslaeva,
  • Z. R. Khasanshina,
  • M. D. Bochkareva,
  • A. A. Filipenko,
  • R. V. Drai

摘要

This study aimed to determine the optimal cultivation conditions for four Escherichia coli strains producing recombinant glucagon-like peptide-1 tandems. Validation experiments confirmed that scale-up from shake flasks to a bioreactor is feasible without a significant loss in specific productivity. We also demonstrated that the optimal cultivation conditions were identical for all tested strains. However, the number of glucagon-like peptide-1 monomers in the tandem affected the final yield in both shake flasks and the bioreactor. We also evaluated the target protein fraction in the wet inclusion bodies obtained. The amount of inclusion bodies per gram of biomass was found to correlate with specific productivity, while the target protein fraction in wet inclusion bodies varied among the different tandems. A correlation was identified between the titer of the glucagon-like peptide-1 tandems and the number of monomers. The results indicate that selecting an appropriate number of monomers during strain development can enhance productivity, improve the target protein fraction in wet inclusion bodies, and increase the final product titer after downstream processing.