<p>Antibiotic-resistant microorganisms are a major concern for researchers, medical experts, and public healthcare workers. Healthcare organizations, particularly wastewater from hospitals, can pose a significant global health risk if wastewater treatment (WWT) strategies are inadequate or suboptimal. This review article focuses on antibiotic-resistant microorganisms and virulence factors found in microbial contaminants of hospital wastewater. In this review, we synthesize findings from a wide range of studies examining antibiotic resistance and virulence factors of microorganisms in hospital wastewater, highlighting the critical public health challenge posed by microbial contamination in healthcare environments. The rise of drug-resistant bacteria represents a severe threat to global health since ailments arising from these organisms are becoming more challenging to cure. Understanding the virulence mechanisms of the aforementioned diseases is essential for developing potent disease-effective prevention and counter-measure strategies. Analysis of hospital effluents reveals a variety of virulence factors, emphasizing the prospective health risks linked with wastewater pollution. The surrounding and societal influence on the well-being of hospital waste underscores the urgent need to develop and implement robust medical waste management and wastewater treatment protocols. This study reviews various treatment technologies aimed at mitigating antibiotic resistance in hospital wastewater, underscoring the importance of comprehensive approaches to curb the spread of drug-resistant bacteria. The insights provided are crucial for improving wastewater management practices to protect public health and prevent the widespread distribution of resistance to antibiotics.</p>