Scholarlink

<p>The objectives of this study were to identify genes associated with vanillin tolerance and to elucidate the link between vanillin tolerance and oxidative stress and the hog1 pathway activation in the yeast <i>Saccharomyces cerevisiae</i> for the production of biofuels and renewable chemicals from lignocellulosic biomass. In this study, one strain tolerant to vanillin stress was isolated through a transposon-mediated mutant library, and the disrupted gene was identified as <i>SAP1</i>. In addition, this phenotype associated with vanillin tolerance was confirmed by deletion and overexpressing of this corresponding gene. In response to vanillin stress, mutant strain was found to reduce intracellular level of reactive oxygen species (ROS), induce activation of Hog1p, and increase expression of the stress-response transcription factors <i>MSN2/4</i> and genes mediated by stress response elements such as <i>CTT1</i>, <i>HSP12</i>, and <i>DDR2</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Disruption of SAP1 confers vanillin tolerance in Saccharomyces cerevisiae

  • 期刊论文

  • 发表日期：

    2025年7月7日

查看全文
  • Bora Kim

摘要

The objectives of this study were to identify genes associated with vanillin tolerance and to elucidate the link between vanillin tolerance and oxidative stress and the hog1 pathway activation in the yeast Saccharomyces cerevisiae for the production of biofuels and renewable chemicals from lignocellulosic biomass. In this study, one strain tolerant to vanillin stress was isolated through a transposon-mediated mutant library, and the disrupted gene was identified as SAP1. In addition, this phenotype associated with vanillin tolerance was confirmed by deletion and overexpressing of this corresponding gene. In response to vanillin stress, mutant strain was found to reduce intracellular level of reactive oxygen species (ROS), induce activation of Hog1p, and increase expression of the stress-response transcription factors MSN2/4 and genes mediated by stress response elements such as CTT1, HSP12, and DDR2.