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<p>The increasing rate of <i>Helicobacter pylori (H. pylori)</i> antibiotic resistance has attenuated the effectiveness of conventional antibiotic-based treatment regimens. This study was aimed at investigating the in vitro inhibitory effects of <i>Lactobacillus reuteri</i> (<i>L. reuteri</i>) strain I300 against <i>H. pylori</i>. The inhibitory effects of live <i>L. reuteri</i> I300 and its different formulations I300L, I300G, and I300T were examined on <i>H. pylori</i> adhesion and invasion to AGS cells. Auto-aggregation and co-aggregation assays and also scanning electron microscopy were performed, evaluating <i>L. reuteri</i> capacity to auto-aggregate and co-aggregate with <i>H. pylori</i>. RT-qPCR and ELISA were used to investigate the expression, and production level of inflammation-related cytokines TNF-α, IL-8, and IL-10. E-cadherin expression level was also measured, determining <i>L. reuteri</i> potential effect on AGS cells integrity. <i>L. reuteri</i> presented a time-dependent capacity to auto-aggregate and co-aggregate with <i>H. pylori</i>. Live <i>L. reuteri</i> and its formulations significantly reduced <i>H. pylori</i> adhesion and invasion of AGS cells. <i>H. pylori</i> treatment with <i>L. reuteri</i> reduced proinflammatory cytokines TNF-α and IL-8 production while increasing anti-inflammatory cytokine IL-10 production. <i>L. reuteri</i> promoted the epithelial cell–cell contact by upregulating E-cadherin expression. This study indicated <i>L. reuteri</i> I300 as a potential probiotic strain with co-aggregation capacity and inhibitory effects against <i>H. pylori</i> adhesion, invasion, and inflammation.</p>

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Inhibitory effects of Lactobacillus reuteri strain I300 against Helicobacter pylori adhesion, invasion, and inflammatory response in gastric epithelial cells in vitro

  • 期刊论文

  • 发表日期：

    2025年4月17日

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  • Ghazaleh Talebi,
  • Ali Nabavi-Rad,
  • Zahra Sadeghloo,
  • Michael Doulberis,
  • Mohammad Reza Zali,
  • Abbas Yadegar

摘要

The increasing rate of Helicobacter pylori (H. pylori) antibiotic resistance has attenuated the effectiveness of conventional antibiotic-based treatment regimens. This study was aimed at investigating the in vitro inhibitory effects of Lactobacillus reuteri (L. reuteri) strain I300 against H. pylori. The inhibitory effects of live L. reuteri I300 and its different formulations I300L, I300G, and I300T were examined on H. pylori adhesion and invasion to AGS cells. Auto-aggregation and co-aggregation assays and also scanning electron microscopy were performed, evaluating L. reuteri capacity to auto-aggregate and co-aggregate with H. pylori. RT-qPCR and ELISA were used to investigate the expression, and production level of inflammation-related cytokines TNF-α, IL-8, and IL-10. E-cadherin expression level was also measured, determining L. reuteri potential effect on AGS cells integrity. L. reuteri presented a time-dependent capacity to auto-aggregate and co-aggregate with H. pylori. Live L. reuteri and its formulations significantly reduced H. pylori adhesion and invasion of AGS cells. H. pylori treatment with L. reuteri reduced proinflammatory cytokines TNF-α and IL-8 production while increasing anti-inflammatory cytokine IL-10 production. L. reuteri promoted the epithelial cell–cell contact by upregulating E-cadherin expression. This study indicated L. reuteri I300 as a potential probiotic strain with co-aggregation capacity and inhibitory effects against H. pylori adhesion, invasion, and inflammation.