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<p>The present work was taken to optimize the decolorization of azo dyes in textile wastewater. <i>Pseudomonas putida</i> was selected due to its high azo bond degradation capability and non-pathogenic nature. The effect of operational conditions in the form of 25 different sets of experiments was applied, and the parameters were categorized in 8 different combinations. Agitation, filamentous fungi, flotation support (Liege or packing), and light’s effect on bacterial decolorization were determined by the method of experimental design. The performance of the chosen microorganism was studied in terms of absorbance of color and chemical oxygen demand (COD) removal. This bacterium was able to decolorize 92% of Reactive Blue 40, 82% of Reactive Yellow 174, and 73% of Reactive Red 220 in 7&#xa0;days. The COD decrement for 7&#xa0;days was determined to be 23%, 19%, and 17% for Reactive Blue 40, Reactive Yellow 174, and Reactive Red 220, respectively.</p>

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Comparative Study of Decolorization Azo-Reactive Dyes by Pseudomonas putida Bacteria

  • 期刊论文

  • 发表日期：

    2025年10月9日

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  • Lotfi Harrabi,
  • Noureddine Baaka,
  • Néji Ladhari

摘要

The present work was taken to optimize the decolorization of azo dyes in textile wastewater. Pseudomonas putida was selected due to its high azo bond degradation capability and non-pathogenic nature. The effect of operational conditions in the form of 25 different sets of experiments was applied, and the parameters were categorized in 8 different combinations. Agitation, filamentous fungi, flotation support (Liege or packing), and light’s effect on bacterial decolorization were determined by the method of experimental design. The performance of the chosen microorganism was studied in terms of absorbance of color and chemical oxygen demand (COD) removal. This bacterium was able to decolorize 92% of Reactive Blue 40, 82% of Reactive Yellow 174, and 73% of Reactive Red 220 in 7 days. The COD decrement for 7 days was determined to be 23%, 19%, and 17% for Reactive Blue 40, Reactive Yellow 174, and Reactive Red 220, respectively.