<p>The current study outlines the use of a bioreduction process to synthesize copper nanoparticles. Copper sulfate pentahydrate serves as a precursor salt, while lemon leaf extract acts as an eco-friendly bio-reducing agent for synthesizing copper nanoparticles. The formation of copper nanoparticles was characterized by UV–visible spectroscopy, Transmission Electron Microscopy (TEM), and Attenuated Total Reflectance–Fourier-Transform Infrared spectroscopy (ATR-FTIR). UV–visible spectroscopy revealed surface plasmon resonance at 320&#xa0;nm. The size and shape of the CuNPs were evaluated through TEM investigations, which showed that the typical particle size ranged from 3 to 11&#xa0;nm. The minimum inhibitory concentration of the biosynthesized CuNPs was analyzed using the broth dilution method against four microbial cultures: <i>Staphylococcus</i> <i>aureus</i> ATCC 6835, <i>Escherichia</i> <i>coli</i> ATCC 8739, <i>Klebsiella</i> <i>pneumoniae</i> ATCC 4352, and <i>Pseudomonas</i> <i>aeruginosa</i> ATCC 10145. Using the Pad-Dry-Cure technique, these biosynthesized CuNPs were applied to cotton fabric. The developed CuNP-finished cotton fabrics were then evaluated in terms of SEM–EDX, antimicrobial activity, in vitro cytotoxicity (direct method), and UV protection. The antimicrobial activity showed 99.93% antiviral effectiveness against the SARS-CoV-2 virus and over 95% bacterial reduction against <i>Klebsiella</i> <i>pneumoniae</i> and <i>Staphylococcus</i> <i>aureus</i> after 20 washes. The cytotoxicity and UV protection results for the CuNP-finished fabric indicated mild cytotoxicity and very good UV-protective properties, ensuring the commercial viability of the finished fabric for various applications.</p>

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A Green Method of Developing Copper Nanoparticle-Based Antiviral Textiles

  • Smita Deogaonkar-Baride,
  • Tanushree Tandel,
  • Anupama Chandel

摘要

The current study outlines the use of a bioreduction process to synthesize copper nanoparticles. Copper sulfate pentahydrate serves as a precursor salt, while lemon leaf extract acts as an eco-friendly bio-reducing agent for synthesizing copper nanoparticles. The formation of copper nanoparticles was characterized by UV–visible spectroscopy, Transmission Electron Microscopy (TEM), and Attenuated Total Reflectance–Fourier-Transform Infrared spectroscopy (ATR-FTIR). UV–visible spectroscopy revealed surface plasmon resonance at 320 nm. The size and shape of the CuNPs were evaluated through TEM investigations, which showed that the typical particle size ranged from 3 to 11 nm. The minimum inhibitory concentration of the biosynthesized CuNPs was analyzed using the broth dilution method against four microbial cultures: Staphylococcus aureus ATCC 6835, Escherichia coli ATCC 8739, Klebsiella pneumoniae ATCC 4352, and Pseudomonas aeruginosa ATCC 10145. Using the Pad-Dry-Cure technique, these biosynthesized CuNPs were applied to cotton fabric. The developed CuNP-finished cotton fabrics were then evaluated in terms of SEM–EDX, antimicrobial activity, in vitro cytotoxicity (direct method), and UV protection. The antimicrobial activity showed 99.93% antiviral effectiveness against the SARS-CoV-2 virus and over 95% bacterial reduction against Klebsiella pneumoniae and Staphylococcus aureus after 20 washes. The cytotoxicity and UV protection results for the CuNP-finished fabric indicated mild cytotoxicity and very good UV-protective properties, ensuring the commercial viability of the finished fabric for various applications.