<p>Natural dyes have been used in textile dyeing for thousands of years. Eco-friendly nature, beautiful and attractive colors, and economic issues have resulted in the significance of using natural dyes. The purpose of this study is to investigate the coloring ability and antibacterial properties of rose water waste from rose water extraction on silk and wool yarns. In addition, the color strength of residue of rose petals extraction was compared with the original ones. Residue of rose petals is a waste material that is produced in large volumes during rose water extraction operations, and its disposal imposes significant costs on the rose water industry. In this study, in addition to evaluating the color characteristics and light fastness of silk and wool dyed with the aqueous and alcoholic extracts of residue of rose petals, the antibacterial effect of these extracts on two Gram-positive <i>Staphylococcus aureus</i> and Gram-negative <i>Escherichia coli</i> bacteria was also investigated. The results showed that all dyed samples had positive <i>a</i>^* and <i>b</i>^* values, indicating their red and yellow hues. According to the findings of this study, the use of color extracts derived from rose water waste can create suitable coloring power and overall fastness on both natural yarns. Furthermore, the results of the antibacterial evaluation proved that this dye can be a promising factor in preventing infectious diseases caused by <i>E. coli</i> and <i>S. aureus</i> bacteria.</p>