<p>Low-level vibration exposures are known to affect multiple tissues in living organisms. This study investigates the effects of vibration exposure on bone tissue in larval zebrafish (<i>Danio rerio</i>). Larvae were exposed to vibrations (44–50&#xa0;Hz) for 24&#xa0;h, with or without anaesthesia, and were compared to controls. Ossification levels of the larval skeleton together with the expression of <i>colX</i> (a marker of bone development) in the operculum bone was used to determine the morphological response to vibrations. Gene expression of bone-related (<i>acp5a</i>,<i> bglap</i>,<i> rankl</i>) and apoptosis-related markers (<i>bax</i>) was then evaluated via RT-qPCR after the 24-hour exposure as well as one week after treatment. Results showed no significant differences in overall ossification or <i>colX</i> expression due to vibration. However, gene expression analyses revealed transient changes: all genes were affected immediately after vibration in contrast to one week later, when only <i>acp5a</i> and <i>bglap</i> were affected. These findings suggest that while short-term low-frequency vibration does not visibly affect bone morphology, it can influence bone homeostasis at the molecular level. Our study also underscores the need for appropriate vibration controls in ground-based simulated microgravity studies in order to assess the impact of vibrations from equipment used (e.g. incubators, or other platforms), particularly in gene expression experiments.</p>