<p>Changes in a cell phenotype occur under the influence of external and internal stimuli of a biochemical and/or physical nature, including gravity. This study is devoted to a comparative analysis of the characteristic reactions of breast cancer cells under different gravitational conditions using the statistical-thermodynamic model of their deformation caused by the orientation properties of the actin cytoskeleton of eukaryotic cells using the actin filament orientation parameter. The free energy form of the cell cytoskeleton is determined followed by a derivation of the evolution equation. The statistical-thermodynamic model describes the basic mechanical behavior of eukaryotic cells, including their viscoelasticity, power-law stress relaxation and fluidization under loading. Numerical modeling of cell cytoskeleton reactions to gravitational effects of different magnitudes, including values of 10^–6∙g and 1∙g, was performed. A comparative analysis of cytoskeleton deformation patterns was carried out based on which the peculiarities of the influence of gravity on the mechanical behavior of cells were established.</p>