<p>Threaded fasteners are used in industrial field. The loosening failure and fatigue failure often occur under long-term external forces, leading to accidents. At present, the permanent, mechanical and frictional anti-loosening are commonly used methods. Compared to ordinary fasteners, their tightening process is more cumbersome, and generates a small amount of stress concentration to reduce fatigue life. At present, the main focus is on improving the tensile strength of bolts and increasing the cost. At the same time, the problem of easy fracture of the first turn of contact between bolt-nut threads cannot be solved. In this study, a novel flexible anti-loosening &amp; fatigue resistance method is proposed, which has resulted in the fastener adopting a similar tightening process as ordinary fastener, exhibiting commendable resistance to loosening, without generating excessive stress concentration, while also improving fatigue resistance. Through finite element analyses and experiments, the anti-loosening &amp; fatigue resistance mechanism of this method is elucidated. This method can resist the sliding between bolt-nut threads when transvers force occurs at bearing surface, at the same time, axial vibration is reduced. Fasteners designed based on this method is suitable for application in modern industry.</p>