<p>In this study, we developed a lateral flow immunoassay (LFIA) for detecting duck adulteration in meat products. The assay employs a sandwich format targeting the molecular biomarker duck immunoglobulin (IgY) for accurate species identification. The system demonstrates high specificity and sensitivity, with no observed cross-reactivity among six commonly encountered mammalian and avian species. It is capable of detecting duck meat adulteration at levels as low as 1% (w/w) in lamb or beef products, and the duck IgY remains detectable even in heat-processed samples. In addition, a rapid extraction device was developed, enabling the entire workflow, including sample pretreatment, to be completed within 10&#xa0;min. Analysis of commercial meat products, further confirmed by qPCR, demonstrated that this LFIA delivers accurate results with high specificity and sensitivity, combined with ease of operation. These attributes make it well suited for rapid on-site screening of duck adulteration in meat products, both raw and processed.</p>

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Rapid Detection of Duck Adulteration in Raw and Cooked Meat Products by Lateral Flow Immunoassay

  • Luan Li,
  • Juan Zhang,
  • Yanger Liu,
  • Xiaoyun Sun,
  • Yuhang Fan,
  • Wenyi Tian,
  • Jianzhu Wang,
  • Wan Dalila Wan Chik,
  • Ailiang Chen

摘要

In this study, we developed a lateral flow immunoassay (LFIA) for detecting duck adulteration in meat products. The assay employs a sandwich format targeting the molecular biomarker duck immunoglobulin (IgY) for accurate species identification. The system demonstrates high specificity and sensitivity, with no observed cross-reactivity among six commonly encountered mammalian and avian species. It is capable of detecting duck meat adulteration at levels as low as 1% (w/w) in lamb or beef products, and the duck IgY remains detectable even in heat-processed samples. In addition, a rapid extraction device was developed, enabling the entire workflow, including sample pretreatment, to be completed within 10 min. Analysis of commercial meat products, further confirmed by qPCR, demonstrated that this LFIA delivers accurate results with high specificity and sensitivity, combined with ease of operation. These attributes make it well suited for rapid on-site screening of duck adulteration in meat products, both raw and processed.