Overview and Perspectives on the Application of QuEChERS for the Analysis of Phenolic Compounds in Food Matrices
摘要
QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) has emerged as a powerful and adaptable sample preparation strategy for the determination of phenolic compounds in food matrices, extending well beyond its original application in pesticide residue analysis. This review provides a critical and systematic assessment of the application of QuEChERS for phenolic analysis in foods, encompassing fruits, vegetables, beverages, honey, and processed products. Emphasis is placed on methodological variables that directly affect extraction efficiency and analytical reliability, including sample pre-treatment, solvent selection, partitioning salts, clean-up sorbents, and chromatographic systems. Acetonitrile-based extraction combined with salting-out partitioning and dispersive solid-phase extraction remains the dominant approach; however, matrix-dependent optimization is essential to ensure adequate recovery and minimize the co-extraction of interferents. Phenolic acids and flavonoids are the most extensively studied classes, whereas other bioactive phenolics, such as anthocyanins and volatile phenolic compounds, remain insufficiently explored. Liquid chromatography, particularly when coupled with mass spectrometry, is the prevailing analytical platform due to its sensitivity and selectivity, although diode-array detection continues to represent a viable alternative for routine applications. Overall, this review underscores the robustness and versatility of QuEChERS for food phenolic analysis while highlighting the need for future advances focused on the development of greener and safer extraction solvents and sorbents, method miniaturization to reduce sample and reagent consumption, and the expansion of analytical scope toward a broader diversity of phenolic classes and food matrices.