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<p>The concept of blending oils has emerged as a rational approach to formulate oils with optimized fatty acid ratios, superior stability, and improved nutritional and functional properties. This study aimed to develop a stable oil blend of eri pupal oil (EPO), a sustainable source of bioactive lipids with significant therapeutic benefits and flaxseed oil (FO), and to characterize it using Fourier-transform infrared spectroscopy (FTIR), gas chromatography–mass spectroscopy (GC-MS), and antimicrobial assays. FTIR analysis confirmed the presence of key functional groups such as carbonyl (C=O), alkene (=CH), and methylene (CH<sub>2</sub>), providing insights into the structural composition of the blend. GC-MS analysis demonstrated a reduction of α-linolenic acid (ALA) from 83.89% to 76.92%, with increases in palmitic acid from 7.76% to 16.62% and oleic acid from 0.93% to 2.49%, reflecting significant alterations in the fatty acid profile that may influence nutritional quality, oxidative stability, and functional properties. The antimicrobial efficacy was assessed against <i>Staphylococcus aureus</i>, <i>Salmonella</i> typhi, <i>Candida albicans</i>, and <i>Aspergillus niger</i> via the disc diffusion assay, while the minimum inhibitory concentration was determined by the broth dilution method. Antimicrobial assays revealed the strongest inhibitory activity against <i>A. niger</i>, with a zone of inhibition measuring 9.33 ± 2.31&#xa0;mm and a minimum inhibitory concentration of 20% (<i>v</i>/<i>v</i>). These findings highlight the potential of insect oil–based blends as functional ingredients for future nutraceutical and pharmaceutical applications.</p> Graphical Abstract <p></p>

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A Novel Eri (Philosamia ricini)-Flaxseed (Linum usitatissimum) Oil Blend: Advanced Analytical Characterization by GC–MS and FTIR Spectroscopy Coupled With Multivariate Analysis and In Vitro Antimicrobial Assays

  • 期刊论文

  • 发表日期：

    2026年2月18日

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  • Shreya Srivastava,
  • Akash Mishra,
  • Venkatesh Kumar R.

摘要

The concept of blending oils has emerged as a rational approach to formulate oils with optimized fatty acid ratios, superior stability, and improved nutritional and functional properties. This study aimed to develop a stable oil blend of eri pupal oil (EPO), a sustainable source of bioactive lipids with significant therapeutic benefits and flaxseed oil (FO), and to characterize it using Fourier-transform infrared spectroscopy (FTIR), gas chromatography–mass spectroscopy (GC-MS), and antimicrobial assays. FTIR analysis confirmed the presence of key functional groups such as carbonyl (C=O), alkene (=CH), and methylene (CH2), providing insights into the structural composition of the blend. GC-MS analysis demonstrated a reduction of α-linolenic acid (ALA) from 83.89% to 76.92%, with increases in palmitic acid from 7.76% to 16.62% and oleic acid from 0.93% to 2.49%, reflecting significant alterations in the fatty acid profile that may influence nutritional quality, oxidative stability, and functional properties. The antimicrobial efficacy was assessed against Staphylococcus aureus, Salmonella typhi, Candida albicans, and Aspergillus niger via the disc diffusion assay, while the minimum inhibitory concentration was determined by the broth dilution method. Antimicrobial assays revealed the strongest inhibitory activity against A. niger, with a zone of inhibition measuring 9.33 ± 2.31 mm and a minimum inhibitory concentration of 20% (v/v). These findings highlight the potential of insect oil–based blends as functional ingredients for future nutraceutical and pharmaceutical applications.

Graphical Abstract