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<p>The rapid and accurate detection of foodborne pathogens, particularly <i>Salmonella</i> Typhimurium (<i>S.</i> Typhimurium), is essential for ensuring food quality and safety. This work presents a portable, label-free potentiometric aptasensor using an ion flux polymer membrane for the sensitive and rapid detection of <i>S.</i> Typhimurium in meat and water, operated via a smartphone-based potentiostat. The sensor employs a specific aptamer immobilized on a solid-state contact ion-selective electrode, featuring a cationic marker and a carboxylated poly(vinyl chloride) membrane modified on a laser-induced graphene electrode. Binding of outer membrane protein D from <i>S.</i> Typhimurium to its aptamer hinders cation flux across the membrane, generating measurable potential changes that enable quantitative detection within 8&#xa0;min. The assay offered a linear range of 1–10,000&#xa0;CFU/mL, a detection limit of 1&#xa0;CFU/mL, and a sensitivity of 16.572&#xa0;mV/decade. Sensor performance was validated in various meat and water matrices, including groundwater, tap water, drinking water, pork, and chicken, yielding results consistent with ISO 19250:2010 and a commercial lateral flow immunoassay. Recovery rates from spiked meat samples ranged from 96 to 108%, with relative standard deviations below 5.7%, demonstrating good accuracy in complex matrices. The aptasensor also exhibited excellent specificity toward <i>S.</i> Typhimurium and retained over 90% of its sensitivity after three weeks of storage, confirming its good storage stability and practical applicability. These findings indicate that the proposed portable aptasensor is well suited for routine on-site detection of <i>S.</i> Typhimurium in food products and represents a valuable tool for rapid food safety monitoring.</p> Graphical Abstract <p></p>

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Portable Potentiometric Aptasensor Using an Ion Flux Polymer Membrane for Rapid Detection of Salmonella Typhimurium in Meat and Water

  • 期刊论文

  • 发表日期：

    2026年2月4日

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  • Pattarachaya Preechakasedkit,
  • Chaiwat Pulsrikarn,
  • Suphachai Nuanualsuwan,
  • Chalinee Auanphui,
  • Charles S. Henry,
  • Daniel Citterio,
  • Nipapan Ruecha

摘要

The rapid and accurate detection of foodborne pathogens, particularly Salmonella Typhimurium (S. Typhimurium), is essential for ensuring food quality and safety. This work presents a portable, label-free potentiometric aptasensor using an ion flux polymer membrane for the sensitive and rapid detection of S. Typhimurium in meat and water, operated via a smartphone-based potentiostat. The sensor employs a specific aptamer immobilized on a solid-state contact ion-selective electrode, featuring a cationic marker and a carboxylated poly(vinyl chloride) membrane modified on a laser-induced graphene electrode. Binding of outer membrane protein D from S. Typhimurium to its aptamer hinders cation flux across the membrane, generating measurable potential changes that enable quantitative detection within 8 min. The assay offered a linear range of 1–10,000 CFU/mL, a detection limit of 1 CFU/mL, and a sensitivity of 16.572 mV/decade. Sensor performance was validated in various meat and water matrices, including groundwater, tap water, drinking water, pork, and chicken, yielding results consistent with ISO 19250:2010 and a commercial lateral flow immunoassay. Recovery rates from spiked meat samples ranged from 96 to 108%, with relative standard deviations below 5.7%, demonstrating good accuracy in complex matrices. The aptasensor also exhibited excellent specificity toward S. Typhimurium and retained over 90% of its sensitivity after three weeks of storage, confirming its good storage stability and practical applicability. These findings indicate that the proposed portable aptasensor is well suited for routine on-site detection of S. Typhimurium in food products and represents a valuable tool for rapid food safety monitoring.

Graphical Abstract