<p>Foodborne illnesses are a major threat to global food safety, causing approximately 600 million illnesses and 420,000 deaths per year globally, and therefore, the development of a rapid and sensitive detection technology is essential to ensure public safety. Here, we developed an electrochemical sensor based on PCN-224 MOF nanomaterials for the detection of pathogenic bacteria in food. By using PCN-224 MOFs, which have high porosity and large specific surface area, as a substrate material, the properties of its MOFs material can be effectively loaded with other substances to improve the electrical signal response. Meanwhile, PCN-224 has a large number of carboxyl groups on it, and EDC/NHS was used to activate its carboxyl groups, which in turn coupled with the amino group of ampicillin to load ampicillin onto the electrode in large quantities. The microstructures and elemental compositions of PCN-224 were analyzed using the relevant instruments, and under the optimal conditions, the strategy exhibited a high degree of efficiency against S. aureus with a range of 2.05 × 10⁵ ~ 2.05 × 10¹⁰&#xa0;CFU&#xa0;mL⁻¹ with a low detection limit of 1.34 × 10⁵&#xa0;CFU&#xa0;mL⁻¹, and for <i>E. coli</i>, the strategy exhibited a detection range of 3.91 × 10⁶ ~ 3.91 × 10¹⁰&#xa0;CFU&#xa0;mL⁻¹ with a LOD of 1.66 × 10⁶. In addition, the excellent stability, anisotropy, and reproducibility of our developed sensors provide an innovative approach for the detection of pathogenic bacteria in food and enable the detection of a wide range of pathogens, which is very promising for the detection of foodborne pathogens.</p>