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<p>The presence of β-lactoglobulin (β-Lg), one of the major food allergens, poses a significant health risk to allergic populations. Therefore, the development of a method for the rapid detection of β-Lg is particularly urgent, aiming at the effective prevention of adverse reactions in allergic patients after exposure to or ingestion of foods containing this allergen. In this study, a lateral flow immunoassay (LFIA) using a double-antibody sandwich immunochromatography was developed, with colloidal gold-labeled mouse anti-β-Lg monoclonal antibody 1 (mAb1) as the capture antibody and mouse anti-β-Lg monoclonal antibody 2 (mAb2) as the detection antibody. After optimizing the preparation methods for both the signal probe and the immunochromatographic test strip, the developed test strip exhibited high specificity, high sensitivity (0.5&#xa0;µg/mL), ease of operation, and portability, enabling the rapid and sensitive detection of cow’s milk β-Lg allergen in food samples within 5&#xa0;min. By using β-Lg extraction solution and immunochromatographic test strip, a rapid test kit suitable for the detection of β-Lg allergen in bread, cookies, candies, rice infant formula, and other foodstuffs was developed, which provided a rapid and simple method for the qualitative detection of β-Lg in foodstuffs.</p>

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Determination of Cow’s Milk β-Lactoglobulin Allergens in Foods Based on a Rapid and Sensitive Immunochromatographic Test Strip Method

  • 期刊论文

  • 发表日期：

    2025年11月18日

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  • Yang Wan,
  • Wenhan Kang,
  • Jincheng Han,
  • Jiukai Zhang,
  • Ning Yu,
  • Ying Chen

摘要

The presence of β-lactoglobulin (β-Lg), one of the major food allergens, poses a significant health risk to allergic populations. Therefore, the development of a method for the rapid detection of β-Lg is particularly urgent, aiming at the effective prevention of adverse reactions in allergic patients after exposure to or ingestion of foods containing this allergen. In this study, a lateral flow immunoassay (LFIA) using a double-antibody sandwich immunochromatography was developed, with colloidal gold-labeled mouse anti-β-Lg monoclonal antibody 1 (mAb1) as the capture antibody and mouse anti-β-Lg monoclonal antibody 2 (mAb2) as the detection antibody. After optimizing the preparation methods for both the signal probe and the immunochromatographic test strip, the developed test strip exhibited high specificity, high sensitivity (0.5 µg/mL), ease of operation, and portability, enabling the rapid and sensitive detection of cow’s milk β-Lg allergen in food samples within 5 min. By using β-Lg extraction solution and immunochromatographic test strip, a rapid test kit suitable for the detection of β-Lg allergen in bread, cookies, candies, rice infant formula, and other foodstuffs was developed, which provided a rapid and simple method for the qualitative detection of β-Lg in foodstuffs.