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<p>This study proposes a suspension analysis approach for determining total carbohydrates in beans using spectrophotometry. Although previous studies have quantified carbohydrates using similar colorimetric reactions—typically after hydrolysis or extraction steps—this method employs a minimal sample preparation strategy, applied to intact bean matrices, eliminating the need for time-consuming procedures. Thirty-six bean samples were processed through grinding and sieving before suspension in water, followed by a colorimetric reaction with sulfuric acid and phenol. Validation results like linearity (correlation coefficient of 0.9995), limit of quantification (5.3%), accuracy (comparison with four reference materials), and precision (relative standard deviation up to 10%) were satisfactory. A comparison between the determined carbohydrate levels and those indicated on the bean packaging revealed consistency for most samples analyzed. The contribution of beans to the Recommended Dietary Allowance of carbohydrates (12–13%) showed the importance of grain in the intake of this macronutrient, along with other sources of carbohydrates, like rice, corn, and potatoes.</p> Graphical Abstract <p></p>

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Suspension Analysis: an Innovative Approach for the Determination of Total Carbohydrates in Beans by Spectrophotometry

  • 期刊论文

  • 发表日期：

    2025年9月4日

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  • Ryan Guilherme Maurício França da Silva,
  • Micherlane Maria da Silva,
  • Iago José Santos da Silva,
  • Elvis Joacir De França,
  • Maria José da Silva,
  • Mario Takayuki Kato

摘要

This study proposes a suspension analysis approach for determining total carbohydrates in beans using spectrophotometry. Although previous studies have quantified carbohydrates using similar colorimetric reactions—typically after hydrolysis or extraction steps—this method employs a minimal sample preparation strategy, applied to intact bean matrices, eliminating the need for time-consuming procedures. Thirty-six bean samples were processed through grinding and sieving before suspension in water, followed by a colorimetric reaction with sulfuric acid and phenol. Validation results like linearity (correlation coefficient of 0.9995), limit of quantification (5.3%), accuracy (comparison with four reference materials), and precision (relative standard deviation up to 10%) were satisfactory. A comparison between the determined carbohydrate levels and those indicated on the bean packaging revealed consistency for most samples analyzed. The contribution of beans to the Recommended Dietary Allowance of carbohydrates (12–13%) showed the importance of grain in the intake of this macronutrient, along with other sources of carbohydrates, like rice, corn, and potatoes.

Graphical Abstract