Scholarlink

Background <p>The adulteration of marine fish surimi with freshwater fish without consumer disclosure is a growing concern in China. Thus, a rapid detection method is needed for four major freshwater species: black carp (<i>Mylopharyngodon piceus</i>), grass carp (<i>Ctenopharyngodon idella</i>), silver carp (<i>Hypophthalmichthys molitrix</i>), and bighead carp (<i>Hypophthalmichthys nobilis</i>).</p> Methods and Results <p>Mitochondrial NADH dehydrogenase subunit 5 (ND5) sequences were aligned and analyzed to design species-specific primers and probes for a real-time quantitative polymerase chain reaction (qPCR) assay. This method enabled the simultaneous identification of <i>M. piceus</i>, <i>C. idella</i>, <i>H. molitrix</i>, and <i>H. nobilis</i> in marine fish surimi. The designed primers and probes demonstrated high specificity, with no cross-reactivity to 24 other fish species in the qPCR reaction. The detection limits of the method were 0.0005&#xa0;ng μL<sup>−1</sup> for <i>M. piceus</i> and <i>H. nobilis</i> and 0.005&#xa0;ng μL<sup>−1</sup> for <i>C. idella</i> and <i>H. molitrix</i>. The method detected <i>M. piceus</i> at 0.1%, <i>C. idella</i> at 0.01%, and both <i>H. molitrix</i> and <i>H. nobilis</i> at 0.001% in fish mixtures. Among 50 commercial samples, 31 tested positive for one or more of these species.</p> Conclusion <p>The developed qPCR method specifically detects <i>M. piceus</i>, <i>C. idella</i>, <i>H. molitrix</i>, and <i>H. nobilis</i> in marine fish surimi and has potential for use in routine quality control by food regulators.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development of a Real-Time PCR Method for Identifying Four Major Freshwater Fish in Marine Fish Surimi

  • 期刊论文

  • 发表日期：

    2025年8月15日

查看全文
  • Yutong Li,
  • Meng Qu,
  • Enchong Liu,
  • Shuo Wan,
  • Yanhua Jiang,
  • Yingying Guo,
  • Wenjia Zhu,
  • Na Li,
  • Lianzhu Wang,
  • Lin Yao

摘要

Background

The adulteration of marine fish surimi with freshwater fish without consumer disclosure is a growing concern in China. Thus, a rapid detection method is needed for four major freshwater species: black carp (Mylopharyngodon piceus), grass carp (Ctenopharyngodon idella), silver carp (Hypophthalmichthys molitrix), and bighead carp (Hypophthalmichthys nobilis).

Methods and Results

Mitochondrial NADH dehydrogenase subunit 5 (ND5) sequences were aligned and analyzed to design species-specific primers and probes for a real-time quantitative polymerase chain reaction (qPCR) assay. This method enabled the simultaneous identification of M. piceus, C. idella, H. molitrix, and H. nobilis in marine fish surimi. The designed primers and probes demonstrated high specificity, with no cross-reactivity to 24 other fish species in the qPCR reaction. The detection limits of the method were 0.0005 ng μL−1 for M. piceus and H. nobilis and 0.005 ng μL−1 for C. idella and H. molitrix. The method detected M. piceus at 0.1%, C. idella at 0.01%, and both H. molitrix and H. nobilis at 0.001% in fish mixtures. Among 50 commercial samples, 31 tested positive for one or more of these species.

Conclusion

The developed qPCR method specifically detects M. piceus, C. idella, H. molitrix, and H. nobilis in marine fish surimi and has potential for use in routine quality control by food regulators.