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<p>Cellulase production by <i>Trichoderma reesei</i> using bamboo as a solid substrate has not been previously investigated. Thus, the suitability of bamboo as a cellulase production medium in solid-state fermentation was evaluated using <i>T. reesei</i> ATCC56765 (RUT-C30) in this study. On day 7, the cellulase activity (1.9 filter paper unit activity (FPU)/g) with dry bamboo as the medium was lower than that obtained using wheat straw and rice straw solid media. Cellulase activity increased to 3.6 FPU/g in a dry bamboo-wheat bran mixed medium (BWM) at a weight ratio of 1:1 on day 10. Analysis at cultivation temperatures (26&#xa0;°C, 22&#xa0;°C, and 18&#xa0;°C) lower than the optimal temperature (30&#xa0;°C) revealed the highest cellulase activities of 4.0 and 6.7 FPU/g-dry medium at 22&#xa0;°C in bamboo and BWM, respectively. Furthermore, the highest β-glucosidase activity of 49.3 U/g-medium was achieved at 22&#xa0;°C in BWM, which was 13.3-fold higher than that obtained in bamboo medium at 30&#xa0;°C. Finally, the cellulases produced from the bamboo and BWM media could successfully hydrolyze alkali-treated rice straw and produce ethanol via simultaneous saccharification and fermentation. In conclusion, bamboo represents a suitable medium for cellulase production, and a temperature of 22&#xa0;°C enhances cellulase production with increased β-glucosidase activity.</p>

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Cellulase Production in Bamboo Solid Media using Trichoderma reesei RUT-C30 and its Temperature Dependence

  • 期刊论文

  • 发表日期：

    2025年12月10日

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  • Chizuru Sasaki,
  • Maya Sunami,
  • Kazuo Matsuura,
  • Takeshi Omasa

摘要

Cellulase production by Trichoderma reesei using bamboo as a solid substrate has not been previously investigated. Thus, the suitability of bamboo as a cellulase production medium in solid-state fermentation was evaluated using T. reesei ATCC56765 (RUT-C30) in this study. On day 7, the cellulase activity (1.9 filter paper unit activity (FPU)/g) with dry bamboo as the medium was lower than that obtained using wheat straw and rice straw solid media. Cellulase activity increased to 3.6 FPU/g in a dry bamboo-wheat bran mixed medium (BWM) at a weight ratio of 1:1 on day 10. Analysis at cultivation temperatures (26 °C, 22 °C, and 18 °C) lower than the optimal temperature (30 °C) revealed the highest cellulase activities of 4.0 and 6.7 FPU/g-dry medium at 22 °C in bamboo and BWM, respectively. Furthermore, the highest β-glucosidase activity of 49.3 U/g-medium was achieved at 22 °C in BWM, which was 13.3-fold higher than that obtained in bamboo medium at 30 °C. Finally, the cellulases produced from the bamboo and BWM media could successfully hydrolyze alkali-treated rice straw and produce ethanol via simultaneous saccharification and fermentation. In conclusion, bamboo represents a suitable medium for cellulase production, and a temperature of 22 °C enhances cellulase production with increased β-glucosidase activity.