<p>To examine the relationship between chronotype characteristics, anxiety and depression among nursing students. The research was conducted with 246 nursing students in a province located in eastern Turkey. This study was designed as an observational, descriptive correlational study. Research data were collected using the Nursing Student Identification Form, the Morningness-Eveningness Questionnaire (MEQ), the Beck Anxiety Inventory, and the Beck Depression Inventory-II. SPSS 26 program was used to evaluate the research data, and descriptive analyses, independent samples t test, one way ANOVA test and regression analysis were applied. It was determined that 70.7% of the participants were in the intermediate type in terms of chronotype, 52% had severe anxiety, and 51.6% had minimal depression. It was observed that the anxiety and depression levels of the participants differed significantly according to their chronotype characteristics, and there was a negative relationship between chronotype scores and anxiety and depression (R² = 0.095). This study reveals that participants with an evening chronotype in a sample of nursing students are associated with higher levels of anxiety and depression, highlighting chronotype as a measurable factor linked to psychological vulnerability in this particular academic group. The findings provide practical evidence supporting the inclusion of chronotype assessment in nursing education settings to help identify students at risk of anxiety and depression and to inform targeted mental health screening and support strategies.</p>